为嗜杀酵母原生质体融合育种提供了依据。
It provides scientific basis for the breeding of protoplast fusion of killer yeast.
温度明显影响原生质体融合的频率和速度。
Temperature remarkably affected the frequency and the speed of protoplast fusion.
利用双灭活原生质体融合技术获得了苏云金杆菌新菌株。
The inactivated protoplast fusion method was carried out to select new Bacillus thuringiensis strains.
研究了啤酒酵母原生质体融合株GR8的主要发酵特性。
The fermentative characters of the brewer, s yeast protoplast fusant GR8 were studied.
本文研究的是一种通过原生质体融合技术构建出的细胞工程菌。
In this paper, the strain is cell engineering bacteria, which constructed by the way of protoplast fusion.
本文着重介绍了难栽培食用菌原生质体融合技术，并概括了其发展前景。
The protoplast fusion technology of edible fungi culturing difficultly was introduced mainly, and the technology of future development was discussed.
结论：双亲株灭活原生质体融合选育林可霉素高产菌株是值得推广的一种选育方法。
Conclusion: the selection method for Lincomycin - producing strain with stability and high yield by using the died parental strain protoplast fusion is deserved to generalize.
研究了球形红假单胞菌与酿酒酵母菌的原生质体融合条件，并对融合子性能进行了测定。
The results of the protoplast fusion between Rhodopseudomonas spheroides and Sacchaaromyces cerevisiae were studied in this paper.
啤酒酵母原生质体融合株GR8的中试表明：融合株GR8凝絮性较强（本斯值为3.0）；
The pilot scale experiment of protoplast fusion strain GR8 of beer yeast indicated that GR8 has strong flocculation capability (burns value as 3.0);
小孢子技术、体细胞原生质体融合技术等组织培养手段已广泛应用于油菜育种及基础性研究。
Tissue culture, such as microspore culture and embryo technique was widely developed in rapeseed breeding and fundamental studies.
并对大麦小孢子培养，细胞悬浮培养，原生质体培养和原生质体融合研究的最新进展作了简要介绍。
Also, the progresses in researches of barley microspore culture, cell suspension culture, protoplast culture and protoplast fusion are briefly introduced in this paper.
现代育种手段进行果酒酵母的选育方法有诱变育种、杂交育种、原生质体融合、基因克隆和转化等技术。
The breeding methods of fruit wine yeast nowadays cover mutation breeding, cross breeding, protoplast fusion, and genetic cloning and transformation etc.
原生质体融合是转移目标性状，特别是那些多基因控制或尚未克隆目标基因控制的性状的一种很有潜力的方法。
Protoplast fusion is a potential method, by which the target characters, especially ones controlled by multigenes or un-cloned genes, can be transferred.
双亲灭活原生质体融合技术优点较多，是原生质体融合育种中针对无遗传标记同种不同株双亲的一种常用的有效方式。
Inactivated protoplasts fusion method has more advantages in breeding techniques and also it has been used effectively in parents without any heritable labels.
其内容包括利用原生质体融合、基因工程等技术进行菌株遗传改良和利用酶谱分析、分子遗传学等技术研究其遗传变异。
This included genetic improvement of strains by protoplast fusion and molecular biology and studies on genetic variability by zymogram technology and molecular genetics.
农抗5102产生菌同源菌株9011和FR008的原生质体融合重组子SR7能产生1种两亲本所没有的抗细菌活性物质。
The fusion recombinant SR 7 of the antibiotic 5102 producing homogenous strains 90 11 and FR 008 produced a new antibacterial substance the parents could not.
原生质体融合技术是微生物育种的有效方法之一，包括原生质体制备、原生质体融合、原生质体再生及融合子的分离等主要过程。
The protoplast fusion technology is an effective ways of microbial breeding, including main processes of protoplast preparation, protoplast fusion, protoplast regeneration, fusion separation.
在酶的解液中进行消化期间，可以观察到一些原生质体自然融合，融合的原生质体体积大、胞质浓、形状各异。
During digestion in the enzyme solution some spontaneous fusion of protoplasts could be observed and the fused protoplasts were generally large, cytoplasmic dense and varied in shape.
本文综述了近年来世界各国果树原生质体培养和细胞融合的研究进展。
The latest developments on protoplast culture and protoplast fusion are focused in this review.
所得双亲菌株原生质体的融合率为3.31%。
The fusion rate of the inactivated parental protoplasts was 3.31%.
进一步开展苹果原生质体培养融合技术的研究，对于创造新种质材料，促进原生质体技术在苹果品种和砧木遗传改良中的应用将具有重要的意义。
Therefore, it is significant to further study protoplast culture and fusion which could create new germplasm and be useful to genetic improvements of cultivars and rootstocks in apple.
对原生质体的分离、培养、融合及植株再生方面进行了简要的论述。
The isolation, culture and fusion technology of plant protoplast have been developing rapidly over the past twenty years.
综述了微繁、胚培养、胚乳培养、花药培养、原生质体培养与融合、转基因和基因克隆、分子标记等生物技术近年来在柑桔等南方果树上的应用。
This paper summarized the applications of the micro-propagation, embryo culture, protoplasm cultivate and amalgamation, genetic transplant and clone, molecule marking on fruit in south.
电融合后的融合子和原生质体在固体培养基上植板培养成菌落。
The colonies grew up from the fusants or protoplasts plated on solid medium after electrofusion.
电融合后的融合子和原生质体在固体培养基上植板培养成菌落。
The colonies grew up from the fusants or protoplasts plated on solid medium after electrofusion.
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