利用双灭活原生质体融合技术获得了苏云金杆菌新菌株。
The inactivated protoplast fusion method was carried out to select new Bacillus thuringiensis strains.
其内容包括利用原生质体融合、基因工程等技术进行菌株遗传改良和利用酶谱分析、分子遗传学等技术研究其遗传变异。
This included genetic improvement of strains by protoplast fusion and molecular biology and studies on genetic variability by zymogram technology and molecular genetics.
同时，对原生质体再生菌株的形态与多杀菌素产量变化进行了比较研究。
In addition, we compared the change of morphology and spinosyns yield of the regenerated strains.
所得双亲菌株原生质体的融合率为3.31%。
The fusion rate of the inactivated parental protoplasts was 3.31%.
研究了利用珍稀食用菌茶树菇双核菌丝进行原生质体分离和再生，以及原生质体单核菌株的筛选过程。
The preparation of monokaryon protoplasts and the screening of protoplast-derived monokaryon strains are important parts in mushroom research and breeding.
结论：双亲株灭活原生质体融合选育林可霉素高产菌株是值得推广的一种选育方法。
Conclusion: the selection method for Lincomycin - producing strain with stability and high yield by using the died parental strain protoplast fusion is deserved to generalize.
不同菌株原生质体产量差异不显著。
There was also no significant difference in the yield of protoplasts from different strains.
原生质体再生菌株仍保留亲株的菌落形态及合成赤霉素的生产能力。
The yield of Gibberellin and morphology of colonies of the regenerated strains from protoplasts are the same as those of the parent strain.
以香菇菌株精选和武香作为亲本制备单核原生质体，将两个亲本的单核原生质体进行一一杂交。
Protoplasted monokapyon(PM) were prepared from Lentinus edodes strains Jingxuan and Wuxiang, respectively, intercross process were put in practice.
为提高海南霉素产量，分别对其产生菌进行氮离子束注入、原生质体再生等处理，获得高产菌株2-7、Y81。
High producing mutants 2 7 and Y81 of hainanmycin were obtained by ion beam implantation or protoplast regeneration.
以脆壁酵母ry - 3作为出发菌株，对其营养体及原生质体进行紫外线诱变处理。
S. fragilis RY-3 as starter, its cell and protoplast were mutated with ultraviolet radiation.
通过对A4原生质体紫外线诱变筛选得到酒精产量明显提高的酵母菌株。
The yeast increased the ethanol production was screened by UV-mutation of A4 protoplast.
农抗5102产生菌同源菌株9011和FR008的原生质体融合重组子SR7能产生1种两亲本所没有的抗细菌活性物质。
The fusion recombinant SR 7 of the antibiotic 5102 producing homogenous strains 90 11 and FR 008 produced a new antibacterial substance the parents could not.
本文报告食用菌侧耳属四个种六个菌株原生质体分离与再生的试验结果。
This paper reports experimental results of protoplast preparation and regeneration with 6 strains which belong to 4 species within genus pleurotus.
用紫外线照射紫孢侧耳（Pleurotus sapidus）双核菌丝原生质体，再生后筛选生长势好的菌株，通过出菇试验得到了生产性状与紫孢侧耳一致的无孢和少孢平菇新品种。
This paper reported isolation and regeneration of the dikaryocyte protoplasts from Pleurotus sapidus, and the protoplasts were treated by U. V-irradiation for selection spore less mutants.
用紫外线照射紫孢侧耳（Pleurotus sapidus）双核菌丝原生质体，再生后筛选生长势好的菌株，通过出菇试验得到了生产性状与紫孢侧耳一致的无孢和少孢平菇新品种。
This paper reported isolation and regeneration of the dikaryocyte protoplasts from Pleurotus sapidus, and the protoplasts were treated by U. V-irradiation for selection spore less mutants.
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