• 系统建立基础性工作原生质体培养生理研究

    Basic work for the system establishment about protoplast culture and physiological research.

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  • 马铃薯叶肉细胞原生质体培养,再生细胞形成细胞团愈伤组织

    The mesophyll cell protoplasts of potato could divide from cell colonies and callus after cultured.

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  • 本文综述了近年来世界各国果树原生质体培养细胞融合的研究进展

    The latest developments on protoplast culture and protoplast fusion are focused in this review.

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  • 原生质体培养体系建立对各种遗传操作利用生物技术培育新品种具有重要意义

    The formation of the system of protoplast culture has an important significance in various kinds of genetic operations and in utilizing biological technology to cultivate new varieties.

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  • 大麦小孢子培养细胞悬浮培养原生质体培养原生质体融合研究最新进展作了简要介绍

    Also, the progresses in researches of barley microspore culture, cell suspension culture, protoplast culture and protoplast fusion are briefly introduced in this paper.

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  • 对甘薯原生质体培养植株再生、利用原生进行体细胞杂交作为遗传工程受体研究进行了综述

    It is reviewed on protoplast culture plant regeneration, somatic hybridization by protoplast, and protoplast as an acceptor of genetic engineering in this literature.

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  • 目前原生质体培养药用植物高产细胞系筛选、体细胞杂交基因工程方面取得了长足的进展

    Recently, the protoplast culture of medicinal plants has mace great progress in the saltation of high productive cell lines, somatic cell hybridization and gene engineering.

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  • 通过愈伤组织培养悬浮细胞培养原生质体培养方法对草坪草的一些建立较为完善植株再生体系

    The plant regeneration systems of some turfgrass species have been established perfectly through callus culture, cell suspension culture, and protoplast suspension culture.

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  • 红麻器官培养原生质体培养品种繁、变异体筛选以及体细胞杂交和遗传转化等生物技术育种的基础

    It's organ culture and protoplast culture is important basic technique of rapid clonal propagation, selection of imitative plants, somatic hybridization and genetic transformation.

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  • 进一步开展苹果原生质体培养融合技术的研究,对于创造种质材料,促进原生质体技术苹果品种砧木遗传改良中的应用将具有重要的意义。

    Therefore, it is significant to further study protoplast culture and fusion which could create new germplasm and be useful to genetic improvements of cultivars and rootstocks in apple.

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  • 综述繁、培养胚乳培养、花药培养原生质体培养融合、转基因基因克隆分子标记等生物技术近年来柑桔等南方果树应用

    This paper summarized the applications of the micro-propagation, embryo culture, protoplasm cultivate and amalgamation, genetic transplant and clone, molecule marking on fruit in south.

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  • 主要研究结果如下1。研究了小青杨茎段培养愈伤组织再生技术,进一步开展离体叶片细胞染色体加倍以及原生质体培养奠定了基础。

    Results are as follows: 1. Axillary buds culture and callus differentiation were studied in this paper, which provide experience for in vitro chromosome doubling and protoplast culture techniques.

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  • 通过愈伤组织培养悬浮细胞培养原生质体培养手段,对早熟禾黑麦草属、结草属剪股一些物种已建立了较为完善的植株再生体系

    Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture, suspension culture, and protoplast suspension culture.

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  • 原生质体浅层液体培养固体培养进行了比较

    The thin liquid culture was compared with that of the AGAR plate.

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  • 结果表明,离体培养24小时原生质体表面产生一些突起有时可见少量纤维组分的形成

    The results showed that after 24 h in culture a number of protruding vesicles, as well as a small amount of fibrillar component were formed on the surface of protoplasts.

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  • 结果表明:枣叶片分离原生质体活性显著高于悬浮培养物,但是产量低于悬浮培养物分离出原生质体

    The results showed that the viability of protoplasts isolated from leaves was significantly higher than that of the suspension culture, but the yield of protoplasts was just the opposite.

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  • 应用反复冻融法以衣藻原生质体为材料制备衣藻染色质,应用显微操作技术准确将衣藻染色质转移到烟草叶片外植体中,进行连续培养镜检观察

    Then we exactly transformed the chromatin to the explants of the tobacco leaves by the micromanipulation, and then we cultured the explants, observed by the fluorescent microscope every day.

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  • 孢子技术、体细胞原生质体融合技术组织培养手段广泛应用于油菜育种基础性研究

    Tissue culture, such as microspore culture and embryo technique was widely developed in rapeseed breeding and fundamental studies.

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  • 油菜已经建立了子叶胚轴原生质体小孢子培养再生体系

    Rape have established regeneration system of cotyledons, hypocotyls, protoplasts and microspore culture.

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  • 芦笋的花药、花粉原生质体离体培养再生植株

    The anther, pollen, stem point and bioplast cultured in vitro can be plant.

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  • 结果表明,纤维培养双层培养比较适合灵芝原生质体再生

    The results showed that the cellobiose medium and double layer culture method were most suitable for protoplast regeneration of Ganoderma lucidum.

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  • 原生质体培养基中诱导伤组织。

    They were induced to small calli in the medium.

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  • 对鹰嘴紫云英抗性系、半日花、霸王三种植物进行原生质体分离培养条件的研究。

    The conditions of isolation and culture of protoplasts from resistant Astragalus cicer L. cell line, Helianthemum Songaricum Schrenk and Zygophyllum xanthoxylum were studied.

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  • 原生质体分离培养融合植株再生方面进行了简要的论述。

    The isolation, culture and fusion technology of plant protoplast have been developing rapidly over the past twenty years.

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  • 较高渗透压培养培养原生质体再生的细胞团及伤组织,可提高植株再生频率

    Using media with relatively high osmotic pressure in cul-turing protoplast-derived calli increased their plant regeneration frequency on subsequent differentiation media.

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  • 采用麸皮再生培养替代R2YE再生培养基,原生质体再生率、生长及筛选效果得到明显改善。

    The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium.

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  • 培养适于培养大豆等豆科植物未成熟子叶原生质体,通过性愈伤组织,经胚胎发生途径。

    This callus can differentiate somatic embryo and regenerate plant on the culture medium somatic embryo differentiation.

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  • 稳定剂为琥珀酸钠,再生培养方法平板双层法。原生质体形成再生率分别达到93.8%37.5%。

    The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.

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  • 稳定剂为琥珀酸钠,再生培养方法平板双层法。原生质体形成再生率分别达到93.8%37.5%。

    The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.

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