干燥的培养基易导致原球茎及幼苗萎蔫、褐化。
类原球茎诱导分化过程中所需的蔗糖浓度相对较低。
Low level of sucrose is required during induction of Protocorm Like-Body and shoots differentiation.
诱导子处理能促进原球茎中生物碱类成分的产生或特异性积累。
The alkaloids in protocorms could be induced susceptibly to produce and accumulate.
试验结果表明：幼叶与茎尖是蝴蝶兰原球茎诱导的较佳外植体；
The results showed that young leaves and shoot-tips are the best explants for PLB induction.
目的：研究MF24真菌诱导子对铁皮石斛原球茎多糖含量的影响。
The effects of MF24 fungal elicitor on the polysaccharide content of Dendrobium candidum protocorms were studied in this work.
固体培养和液体悬浮培养的石斛原球茎细胞生长曲线大致呈“S”型。
The growth curve of Dentrobium cell showed "s" type when the protocorm were cultured on solid media or with liquid suspension way.
原球茎的增殖、分化与培养基的选择有不同的结论，需要作进一步验证。
There were many different reports on the multiplication and differentiation of protocorm and the choice of culture media, but they need further test and verify in practice.
本实验以大花蕙兰根蔸为外植体，研究以原球茎途径再生植株的方法进行快速繁殖。
This research takes root stumps as explant and adopts the regeneration way of PLB to establish a rapid technique system of micropropagation in Cymbidium Grandiflorium.
不同浓度秋水仙素诱变时间长短与类原球茎细胞内部结构发生变化的程度关系密切；
The time of shoot formation was longer and the rate of shoot formation was lower when the concentration of colchicines got higher or the time of treatment got longer.
试验了基本培养基、蔗糖浓度、天然提取物以及植物激素对铁皮石斛原球茎分化的影响。
Effects of basal culture medium, sucrose concentration, natural extracts and phytohormones on protocorm differentiation were studied.
目的：研究细茎石斛拟原球茎生长规律及其与两种有效成分总生物碱和可溶性多糖积累的关系。
Objective: To study the relationship between growth of Protocorm-like bodies and accumulation of total alkaloids and polysaccharides (two active constituents) in Dendrobium moniliforme.
以蝴蝶兰试管苗茎尖为外植体，接种于添加不同浓度激素配比的1/2 MS培养基上进行原球茎诱导。
The stem tips of phalaenopsis test-tube shoot were used as explants to be cultured in the medium(1/2MS) with different concentrations of hormones.
采用光学显微镜和电子显微镜技术对离体培养条件下卡德丽亚兰原球茎产生及形态建成进行了系统的细胞学观察。
Formation and morphogenesis of the protocorms of Cattleya hybrid were investigated in cytology by light micros copy and electronic microscopy.
这一培养条件下原球茎的总生物碱含量最高(0.068%)，与平均值(0.048 8%)相比提高了39.3%。
By this way, the highest content of total alkaloids of protocorms (0.068 0%) was increased by 39.3% comparing with average value (0.048 8%).
将方法3制备的诱导子以高剂量加入原球茎时，获得的总生物碱含量最高(0.048 6%)，与对照(0.034 9%)相比提高了39.2%。
Compared with control (0.034 9%), the highest content of total alkaloids of protocorms (0.048 6%) treated with elicitor T3 at high dosage was increased by 39.2%.
取继代培养30 d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
取继代培养30 d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
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