目的克隆人TRAIL基因，构建其原核表达载体。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
目的：构建丙型肝炎病毒(HCV)全长及3种不同缺失突变的核心蛋白基因的原核表达载体，并在大肠杆菌中表达。
AIM: to construct the recombinant plasmids expressing full-length HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E. coli.
结论：构建了8r - MUC1核心肽融合蛋白原核表达载体并成功表达与纯化出具有生物学活性的融合蛋白。
Conclusion the prokaryotic expression vector of 8r-muc1 core peptide fusion protein has been constructed, and the fusion protein with biological activity has been successfully expressed and purified.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
完成了NKG2D的原核表达载体的构建，表达并纯化了重组NKG2D蛋白。
The NKG2D prokaryotic expression vector was successfully constructed. The recombinant NKG2D is expressed and purified.
目的构建柔嫩艾美耳球虫子孢子表面抗原原核表达载体，并且在大肠杆菌中表达。
Objective To construct the prokaryotic expression vector of the sporozoite surface antigen gene of Eimeria tenella GZ strain and expression in Escherichia coli.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
结论已成功构建了人hsp70与MAGE - 4抗原表位基因的原核表达载体，为疫苗研究提供了依据。
Conclusion the prokaryotic expression vector for HSP70 and MAGE-4 epitope genes was successfully constructed, which provided a basis for the development of vaccine.
目的构建人胃动素受体基因的原核表达载体。
Objective to construct expression plasmid of human motilin receptor.
目的：构建血管基膜衍生多功能肽克隆和原核表达载体，并对血管基膜衍生多功能肽氨基酸序列进行空间结构分析预测。
Objective: to construct cloning and prokaryotic expression vector of vascular basement membrane-derived multifunctional peptide and to analyze its space conformation.
目的构建结核杆菌分泌蛋白mpt53原核表达载体并进行表达和纯化。
Objective to construct a expression vector of MPT53 of mycobacterium tuberculosis and identify and purify the protein in the e.
目的：构建人热休克蛋白70（HSP 70）的原核表达载体，诱导其表达并纯化。
Objective: To construct the prokaryotic expression vector of human heat shock protein 70(HSP 70) and to induce the expression and purification of HSP 70 in vitro.
将构建好的ABP1及TIR1原核表达载体，转化表达宿主菌bl21 (DE3)，加入诱导物iptg进行诱导表达。
The ABP1 and TIR1 prokaryotic expression vectors were transformed into expression host strain BL21 (DE3), then after, IPTG was added to induce expression.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
结论：原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
构建牛结核分枝杆菌esat - 6基因的原核表达载体，诱导表达、纯化并初步鉴定该蛋白。
To construct the recombinant prokaryotic expression vector containing the ESAT-6 gene of Mycobacterium Boris, purification, expression the fusion protein identified.
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