原核生物的基因与真核生物的基因在组织形式和表达方式方面有哪些主要的区别？
What are the most significant differences between the organization and expression of prokaryotic genes and eukaryotic genes?
这是第一次从原核到真核再到原核生物的跨生物种类的基因组转移。
This is the first time that genomes have been transferred between branches of life—from a prokaryote to eukaryote and back to a prokaryote.
由于生产转基因动物的基本方法如原核注射法等普遍存在外源基因整合的低效性和随机性等问题，因而在应用中受到很大制约。
The basic reliable methods available to produce transgenic animal such as pronuclear microinjection has limited application by low efficiency and mosaic integration into the genome.
新基因进行原核表达并纯化蛋白。
在原核生物的进化过程中，基因的横向转移是一个重要的进化机制。
Horizontal gene transfer is an important evolutionary mechanism in prokaryotic genome evolution and has been widely studied.
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
目的克隆srg基因、原核表达并鉴定。
目的克隆人TRAIL基因，构建其原核表达载体。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
目的克隆与原核表达“牵引丝蛋白基因”单体六聚物，为开展具有不同长度系列的“牵引丝蛋白基因”单体多聚物的功能研究奠定基础。
Objective To clone and express the hexamer of the gene of spider dragline silk protein, as a foundation of further research on the functions of the dragline silk protein gene of different lengths.
目的：克隆人MAGE3基因并进行原核表达和分离纯化。
AIM: to clone human MAGE 3 gene, to induce its prokaryotic expression and to purify the protein.
本论文主要致力于原核生物与真核生物及冠状病毒蛋白质编码基因识别以及基因组分析方面的工作。
This paper describes some new approaches for recognizing protein-coding genes in bacterial and archaeal, coronavirus and eukaryotic genomes by using the Z curve method.
简单重复序列广泛分布于原核和真核基因组。
Simple Sequence Repeat is ubiquitous in prokaryotic and eukaryotic genomes.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
目的：构建丙型肝炎病毒(HCV)全长及3种不同缺失突变的核心蛋白基因的原核表达载体，并在大肠杆菌中表达。
AIM: to construct the recombinant plasmids expressing full-length HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E. coli.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
结论已成功构建了人hsp70与MAGE - 4抗原表位基因的原核表达载体，为疫苗研究提供了依据。
Conclusion the prokaryotic expression vector for HSP70 and MAGE-4 epitope genes was successfully constructed, which provided a basis for the development of vaccine.
它们被认为是某些真核生物基因和原核生物基因的混合体。
They appear to have a mixture of prokaryotic and eukaryotic genes.
目的构建人胃动素受体基因的原核表达载体。
Objective to construct expression plasmid of human motilin receptor.
微卫星DNA是广泛分布于原核、真核生物基因组中的短小串联重复的DNA序列。
Microsatellite DNA is short tandem repeats of DNA sequences, which is widely distributed in prokaryotic and eukaryotic genome.
我们利用基因重组技术构建了MGC5306的原核表达体系，并成功表达了MGC5306 (98- 204aa)。
We use gene recombination technology to build the prokaryotic MGC5306 expression system, and we have successfully expressed MGC5306 (98-204aa).
汪静。李官成。童永清鼻咽癌人源抗独特型基因工程抗体的原核表达及鉴定。
Wang J. Li GC Expression, purification and identification of the human anti-idiotypic single chain antibodies against nasopharyngeal carcinoma in E. coli.
目的：构建食管癌相关基因2ECRG2原核表达质粒，在大肠杆菌e。
Objective: to construct a prokaryotic expression vector containing esophageal cancer related gene 2 ECRG2 and observe its expression in Escherichia coli e.
目的改构高致病性禽流感病毒血凝素基因，建立有效的原核表达体系。
Objective To modify the HA1 gene of high pathogenic avian influenza virus (HPAIV) and to build an efficient prokaryotic expression system of it.
结论原核表达系统可有效克隆较高纯度的CHM - I基因。
Conclusion The more effective CHM-I clone with high purity could be expressed by procaryotic expression system.
论文第四章重点介绍用非线性映射方法分析原核生物基因密码子使用情况并得到的相关结论。
In chapter IV, the codon usage preferences in prokaryotic organism are analyzed by nonlinear mapping method.
原核生物基因水平转移的主要方式有转化、接合和转导，真核生物中水平转移发生方式尚不清楚。
Transformation, conjugation, and transduction are the main forms of horizontal gene transfer in prokaryotes, but no clear clue was related with the mechanism of horizontal gene transfer in eukaryotes.
该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；
The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;
该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；
The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;
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