目的探讨HPV免疫组化技术及原位杂交技术在尖锐湿疣病变中的应用价值。
The objective is to study the value of HPV immunohistochemical technique and in situ hybridization for diagnosis of condylomata.
方法采用免疫组化及原位杂交技术。
Methods Immunohistochemistry and in situ hybridization technique were adopted.
方法:原位杂交技术结合图像分析。
METHODS: In situ hybridization and image -analysis system were used.
方法免疫细胞化学和原位杂交技术。
方法:应用原位杂交技术,检测端粒酶的mRNA。
Methods: telomerase activity was detected by using in situ hybridization mRNA of telomerase.
染色体原位杂交技术是现代生物技术的重要组成部分。
The chromosome in situ hybridization is an important part in modern biotechnology.
使用原位杂交技术,研究员追踪了许多的生长-相关的基因水平。
Using in situ hybridization techniques, the researchers tracked the levels of a number of growth-related genes.
目的建立多色荧光原位杂交技术检测人卵细胞染色体非整倍体的方法。
Objective: To establish the method of aneuploidy detection in human oocytes by multicolor fluorescence in-situ hybridization (M-FISH).
方法建立家兔心肌缺血再灌流损伤模型,通过原位杂交技术观察研究。
Methods A rabbit model of myocardial ischemia and reperfusion injury was established, and HSP70mRNA in myocardium was detected by in situ hybridization histochemistry.
另外,荧光原位杂交技术可以鉴别有关病理学表现的新的循环遗传改变。
In addition, the technique of hybridization in situ with fluorescence allowed him to identify new recurrent genetic changes that are involved in the appearance of this pathology.
这9个类型中有的结合细胞学镜检与核酸原位杂交技术已确定为异附加系。
Some of the 9 types have been identified as alien addition lines through cytology techniques and in situ hybridization of nuclear acids.
方法以原位杂交技术检测36例胃癌组织hpa - m RNA的表达。
Methods 36 cases of GC were examined by in situ hybridization technology.
方法利用免疫组织化学和原位杂交技术显示大鼠心肌组织的I型胶原表达。
Methods Expression of cardiac collagen I was detected using immunohistochemistry and in situ hybridization.
利用RNA原位杂交技术,对水稻受精前后雌蕊组织切片进行磷酸化酶基因表达的定位。
Phosphorylase gene expression was localized in tissue sections of rice pistils before and after fertilization by in situ RNA hybridization.
目的:探讨在低温塑料包埋制备的不脱钙骨切片上运用免疫组化和原位杂交技术的可行性。
Objective To investigate the feasibility of applying immunohistochemistry (IH) and in situ hybridization (ISH) technique to the undecalcified bone sections.
通过靶向测序和荧光原位杂交技术发现,3例均存在ZC 3h7b -BCOR基因融合。
All 3 ESS were found to harbor ZC3H7B-BCOR gene fusions by targeted sequencing and fluorescence in situ hybridization.
方法应用组织病理学、免疫组化、原位杂交技术观察25例鼻淋巴瘤的临床及病理形态学特征。
Methods 25 cases of nasal lymphoma with formalin-fixed and paraffin-embedded biopsy speciments were analyzed by histopathology, immunohistochemistry and in situ hybridization.
抗病易位系Y96060可通过细胞遗传分析,染色体C分带和原位杂交技术,RAPD技术得到确认。
Said disease-resisting translocation line Y96060 can be confirmed by means of cytogenetic analysis, chromosome C zoning and in situ hybridization and RAPD technique.
结论与传统的染色体显带技术相比,荧光原位杂交技术具有高效、灵敏、可靠的特点,可为临床提供良好的辅助诊断。
Conclusions Compared with the conventional chromosome banding technique, FISH has characteristics of high sensitivity and accuracy, and provides a good assist method for diagnosis.
通过粗线期染色体荧光原位杂交技术,将发生易位的第6号和第12号染色体的易位点分别锚定在1个和3个BAC克隆库中。
Transpositional points between chromosome 6 and 12 were anchored in 1 and 3 BAC clone's pool respectively by fluorescence in situ hybridization technology of pachytene chromosomes.
方法联合应用常规细胞遗传学、间期荧光原位杂交技术和多重荧光原位杂交技术对14例伴有复杂核型异常的aml患者进行研究。
Methods Multiplex FISH was used in combination with conventional cytogenetics (CC) and interphase FISH to study 14 cases of AML with complex karyotypic abnormalities.
接下来利用爪蟾胚胎致畸实验(FETAX)、组织切片技术和整体原位杂交技术,研究了三种酰胺类除草剂对热带爪蟾胚胎的致畸效应。
Next, using FETAX test, tissue section and whole mount in situ hybridization technique to study the teratogenic effect of three herbicides on early stages of development of X. tropicalis.
结论原位杂交技术的敏感性及特异性较高,且重复性较好,是研究HCVRNA在组织中定位分布和病毒复制场所一种切实可行的方法。
Conclusion the results suggested that the in situ hybridization technique had high sensitivity, specificity and repeatability, which was practical for studying the location and replication of HCV RNA.
原位杂交组织化学技术。
目的探讨荧光原位杂交(FISH)技术的临床价值。
Objective To explore clinical value of the fluorescent in situ hybridization (FISH) technique.
荧光原位杂交是当前分子细胞遗传学最重要的技术之一,克服了实体瘤经典细胞遗传学研究的主要障碍。
Florescence in situ hybridization (FISH), one of the most important molecular cytogenetic techniques, overcomes the main inconvenience in classical cytogenetic methods in solid tumors.
其技术主要包括胚胎活检、聚合酶链反应及荧光原位杂交。
The basic techniques currently used involve embryo biopsy, the polymerase chain reaction and fluorescence in situ hybridization.
实验采用原位杂交,NADPH -NBT组织化学,RNA斑点印迹及蛋白质斑点印迹技术。
The experiment was performed by using in situ hybridization, NADPH NBT histochemistry, RNA dot blot and protein dot blot techniques.
目的探讨用多色荧光原位杂交(MFISH)技术检测的易位和双着丝粒染色体畸变的差异。
Objective To explore the differences between radiation-induced translocation and dicentric chromosome aberrations detected by multicolor fluorescence in situ hybridization (M-FISH) method.
目的探讨多重荧光原位杂交(M -FISH)技术检测慢性淋巴细胞白血病(CLL)复杂核型异常(CCA)的价值。
Objective To investigate the value of multiplex fluorescence in situ hybridization (M-FISH) in the detection of complex chromosomal abnormalities (CCAs) of chronic lymphocytic leukemia (CLL).
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