选用新生SD大鼠的髁状突软骨作为原代细胞培养的组织来源，建立髁状突软骨细胞有限细胞系；
The primary MCC separated from the mandibular condylar cartilage of six neonatal SD rats were cultured and a finite cell line was established in vitro.
方法：选取10例初诊或复发急性白血病患者的骨髓进行原代细胞培养，取对数生长期细胞接种于6孔板中；
Methods:Selected 10 patients with newly diagnosed or relapsed acute leukemia in patients with primary bone marrow cells were cultured in logarithmic growth phase cells were seeded in 6 well plate.
体外肝细胞模型(主要是原代肝细胞培养)作为一种强有力的体外研究体系被广泛应用于化学异物的肝毒性研究。
In vitro liver cell models (mainly primary hepatocyte cultures), served as the most powerful in vitro research system are widely used for the study of hepatotoxicity of xenobiotics.
方法：原代胚胎鼠皮质神经元细胞培养，细胞毒性试验及生化指标测定，细胞染色，显微镜下观察形态学改变及影像分析。
Methods: Primary embryonic rat cortical neurons culture, cytotoxicity assays and biochemistry detection, cell stain for the neuron morphology in microscopy and image analysis.
酶消化法进行表皮细胞和成纤维细胞培养，绘制表皮细胞原代生长曲线，MTT法测定传代后表皮细胞的增殖。
The Epidermal cell and fibroblasts culture in vitro were conducted by enzyme digestion. Epidermal cell primary culture was described by growth graph and MTT method was used in subculture cell growth.
前言：目的：探讨高效的原代成年大鼠下颌骨成骨细胞培养方法，为进一步的实验研究奠定基础。
Objective: To investigate an efficient method of primary mandibular osteoblasts culture, and establish a foundation for further experimental study.
结果两种方法均能得到乳腺癌原代细胞，组织块培养法的成功率为90.0%，细胞培养法的成功率为20.0%。
Results the breast carcinoma cells could be cultured by these two methods with a success rate of 90% in the tissue culture method and that of 20% in the cell culture method.
结论酶交替消化法原代培养胎鼠成骨细胞，获得的成骨细胞纯度高、数量大，可作为一种相对可靠、有效的原代成骨细胞培养方法。
Conclusion Osteoblasts achieved by this method are pure with large quantities, so this method can be used as a reliable and efficient way of primary culture.
结论酶交替消化法原代培养胎鼠成骨细胞，获得的成骨细胞纯度高、数量大，可作为一种相对可靠、有效的原代成骨细胞培养方法。
Conclusion Osteoblasts achieved by this method are pure with large quantities, so this method can be used as a reliable and efficient way of primary culture.
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