方法:通过建立新生大鼠皮层神经元原代培养,用不同浓度醋酸铅染毒和给予牛磺酸干预,观察神经细胞的生长和存活情况。
Methods:Put taurine and different concentrations of lead acetate in cultures of cortex neurons and observe the length of dendites and the growth activity of neurons.
结论:高温可诱发原代培养神经细胞凋亡。
Conclusions: Hyperthermia may induce apoptosis of neuroepithelium in primary culture.
以15 ~16天孕龄胚胎小鼠大脑皮层神经细胞原代分离培养模型,观察8.6 ~10毫克%的酪氨酸对发育中的脑神经细胞的影响。
Cerebral cortex from 15-16 days old fetal mice was utilized to prepare primary dissociated cell cultures to evaluate the effect of tyrosine (8.6-10mg %) on developing nervous cells.
方法:胚胎大鼠脊髓神经细胞原代培养,倒置相差显微镜下进行细胞记数和显微测量,观察神经元存活和生长分化的状况。
Methods: Using primary nerve cell culture, we observed the survival and growth of spinal cord neurons with phase-contract microscope.
方法建立新生大鼠皮层神经元原代培养技术,加载不同浓度牛磺酸对培养皮层神经元进行干预,观察神经细胞的生长和存活情况。
Methods the technique was builded by new-born rats cortex neuron culture. Different concentration of taurine was added to intervene and to study the culture.
目的:应用新生大鼠原代皮层神经细胞体外培养方法,探讨牛磺酸拮抗锰神经毒性作用的机理。
Objective: to explore the mechanisms of taurine prevent neurotoxicity mediated by manganese in vitro on the primary cultivated cerebral cortical neurons from neonatal Wistar rats.
方法采用人胚脑神经细胞原代培养,接种TCID50的HCMV后用光镜、组织染色观察病变全过程。
Methods The human embryo cerebral neurons were prepared for the primary culture using light microscope, tissue staining after inocula-ting HCMV of TCID50.
方法采用人胚脑神经细胞原代培养,接种TCID50的HCMV后用光镜、组织染色观察病变全过程。
Methods The human embryo cerebral neurons were prepared for the primary culture using light microscope, tissue staining after inocula-ting HCMV of TCID50.
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