观察鼠囊胚在小鼠原代胎儿成纤维细胞饲养层上的生长行为。
To observe the mouse blastocyst growth behavior on primary mouse embryonic fibroblast feeder layers (PMEF).
目的观察柑皮提取物对人增生性瘢痕成纤维细胞增殖以及胶原代谢的影响。
Objective To investigate the effects of citrus reticulata bianco extract on the proliferation and collagen metabolism of fibroblasts from human hypertrophic scar.
方法：人皮肤及瘢痕成纤维细胞原代、代培养。
Methods: Fibroblasts from human skin and hypertrophic scar were primary cultured and subcultured.
酶消化法进行表皮细胞和成纤维细胞培养，绘制表皮细胞原代生长曲线，MTT法测定传代后表皮细胞的增殖。
The Epidermal cell and fibroblasts culture in vitro were conducted by enzyme digestion. Epidermal cell primary culture was described by growth graph and MTT method was used in subculture cell growth.
结果：原代培养的成纤维细胞特征典型，并通过传代培养得到了纯化的成纤维细胞。
Results: The cultured cells showed typical shape and features of fibroblast cell, which were successfully dissociated and purified.
在DMEM培养基中进行了原代及传代培养，对其中的成纤维细胞进行分离纯化。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
在DMEM培养基中进行了原代及传代培养，对其中的成纤维细胞进行分离纯化。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
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