用组织块直接培养法，也可以得到皮肤的原代培养细胞。
The primary culture cells of rabbit skin tissue could be obtained by explant culture too.
说明原代培养细胞在血清中能分化为神经元及神经胶质细胞。
It demonstrated that some of the differentiated cells are neurons and the others were glial cells.
结论脊髓前角原代培养细胞的acp酶反应可较好的代表溶酶体的整体分布。
Conclusion ACP ase reaction of primary cultured cells in anterior horns of spinal cord can well represent the integral distribution of lysosome.
目的探讨脊髓前角原代培养细胞ACP 酶的分布，确定溶酶体形态、整体分布及与其它细胞器之间的相互关系。
Objective To research the distribution of ACP ase in primary cultured cells in anterior horns of spinal cord, determine lysosome appearance, integral distribution and their relation with other cells.
无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
迄今为止我们已取得了突变的原代培养的细胞以及蠕虫”。
To date we have made mutant tissue-cultured cells as well as worms.
目的：探索人脂肪源性基质细胞原代培养方法。
Objective: To establish a primary human adipose-derived stromal cells culture method.
方法以人脐静脉内皮细胞（HUVEC）原代培养模型，MTT法测定紫杉醇对HUVEC增殖的影响；
Methods Human umbilical vein endothelial cells were cultured primarily in vitro and influence of Paclitaxel on HUVEC proliferation was evaluated by MTT method.
目的：探索原代培养大鼠肝细胞的最佳分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
结论添加晶体皮质可提高晶体上皮细胞原代培养成功率。
ConclusionAdding lens cortex can increase the success rate of primary culture of lens epithelial cells.
目的对大肠腺瘤细胞原代培养的方法作进一步的改进。
Objective To improve the primary cell culture methods of colorectal adenomas.
结果成功原代培养了猪眼rpe细胞。
Results Primary porcine RPE cells were cultured successfully.
方法：牛晶体上皮细胞原代培养。
Methods: Bovine lens epithelial cells (BLEC) were cultured in vitro.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
目的研究原代培养的脊髓神经干细胞在小鼠视网膜的整合和分化情况。
Objective to study the integration and differentiation of spinal neural stem cells after transplantation into mice retina.
研究人员通过建立动物肝脏再生模型和肝细胞原代培养模型，逐渐发现多种效应因子。
By study on animal models of liver regeneration and primary hepatocytes culture, investigators have proved many types of effective factors.
同时还建立了原代培养的肿瘤细胞，提升了模型的利用效率。
We further developed the primary cell culture if the tumor cells which would lead to better use of this genetic model.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
结论1、用原代培养的方法培养肾上腺细胞可获成功。
Conclusion: 1, the adrenal cells can be cultured by the methods of primary successfully.
目的以原代培养小鼠骨髓基质细胞为饲养层，探讨精原干细胞能否在该饲养层上生长及生长的影响因素。
To study whether spermatogonial stem cells can grow on the feeder layers of bone marrow stromal cells, and their effecting factors in vitro.
方法：分别采用组织块法、酶消化法、组织块酶消化法进行人牙髓细胞原代培养。
METHOD: Human pulp cells were primary cultured with tissue block method, enzymolytic method, and tissue block enzymolytic method separately.
结果原代培养的细胞ALP染色阳性区域可以达90%以上，传代培养后细胞阳性率为100%。
Results the area positive for ALP staining is no less than 90% in the primary culture and almost 100% in the subculture.
本研究拟建立人嗜铬细胞瘤细胞的原代培养方法。
The study is to establish a method of separating and primarily culturing of human pheochromocytoma cells.
研究芳烃类有机溶剂对原代培养大鼠神经胶质细胞的谷氨酸重摄取的影响。
To study effect of aromatic hydrocarbon solvents on the reuptake of amino acid by primary cultured glia cells.
目的探讨体外原代培养大鼠肝细胞的分离方法。
ObjectiveTo explore the isolating methods of rat hepatocytes for primary culture.
目的：建立大鼠耳蜗感觉上皮细胞(CSEC)的原代培养体系，为研究毛细胞再生的分子机制提供大量来源一致的细胞。
Objective: To further investigate the mechanisms of hair cell generation or regeneration, the primary culture systems of cochlear sensory epithelial cell (CSEC) of rats were established.
目的：探讨温脾汤药物血清对原代培养大鼠系膜细胞增殖的影响。
Objective:To study the effect of Wenpitang pharmacologic serum on proliferation of mesangial cells of rats in vitro.
方法以无血清培养基体外原代培养角质形成细胞。
Methods Human keratinocytes were primarily cultured in vitro with serum free medium.
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