目的：观察睫状神经营养因子(CNTF)对皮质酮致原代培养海马神经元损伤的保护作用并探讨其可能机制。
Objective: To elucidate the protective effects of CNTF on the injury of primary cultured cortical and hippocampal neurons induced by corticosterone and its possible mechanism.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
目的研究氟对原代培养大鼠海马神经元氧化应激和DNA损伤的影响。
Objective To explore the effects of fluoride on oxidative stress and DNA damage in primary hippocampal neurons of rats.
以原代培养的大鼠海马神经元为标本，运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。
Methods Cell-attached mode of patch clamp technique were employed to record the single channel current of NMDA receptor with the primary-cultured hippocampal neurons from rats as samples.
目的：利用原子力显微技术（AFM）观察原代培养的海马神经元超微结构及其相互间的连接结构。
Objective To investigate the ultrastructure and the connection structures between primarily cultured hippocampal neurons with atomic force microscopy AFM .
表明本分离及原代培养方法，有益于大鼠海马锥体神经元生长，并适用于膜片钳记录。
The methods of isolation and primary cultivation of hippocampal pyramidal neurons of SD rats improved the growth of neurons and so might be used in patch-clamp recording.
方法 ：应用血清药理学和电生理方法 ，观察六味地黄汤含药血清对原代培养大鼠海马神经元自发突触活动的影响。
The mutagenic activity of the raw water, the effluent of biopretreatment, conventional process, combined process and the four chlorinated counterparts were investigated.
方法 ：应用血清药理学和电生理方法 ，观察六味地黄汤含药血清对原代培养大鼠海马神经元自发突触活动的影响。
The mutagenic activity of the raw water, the effluent of biopretreatment, conventional process, combined process and the four chlorinated counterparts were investigated.
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