方法采用小鼠卵母细胞体外培养、体外受精的方法研究了硫酸锰对小鼠卵母细胞成熟和受精能力的影响。
Methods The effect of manganese vitriol on meiotic maturation and fertilization ability of mouse oocyte was studied by culture of mouse oocyte and in vitro fertilization (IVF).
目的提高人未成熟卵母细胞体外培养的成熟率。
Objective To promote the maturation rate of human immature oocytes in vitro.
免疫活性肽(ICP)和表皮生长因子(EGF)是两种生物活性因子，ICP对牛卵母细胞体外成熟及胚胎体外培养的影响还未见报道。
Immunocompentent peptide (ICP) and epidermal growth factor (EGF) are two bio - active factors, the effect of ICP on in vitro maturation of bovine oocytes and development of embryos not being reported.
卵母细胞体外成熟培养技术是现代生物技术中的重要内容之一。
Occyte maturation culture technology in vitro is one of the main contents in modern biotechnology.
研究结果证明，牛卵巢卵母细胞可作超低温冷冻长期保存，解冻后可以体外培养成熟。
These results indicate that bovine follicular oocytes can be frozen for long term preservation in liquid nitrogen and mature in vitro after thawing.
猪卵母细胞的体外成熟培养、成熟卵母细胞的孤雌激活和胚胎培养是胚胎生物工程技术的主要环节。
The culture of porcine oocytes maturation in vitro, parthenogenetic activation of MII porcine oocytes and embryo culture are main technology of embryo biology engineering.
虽然牛卵母细胞体外成熟培养技术已趋于成熟和完善，但卵母细胞成熟机理的研究还有待深入。
Although the technology of In Vitro maturation is more and more maturated, the mechanism of maturation needs further study.
结果表明海狸鼠卵母细胞与其它啮齿动物的卵母细胞一样，能够在体外培养成熟，完成第一次减数分裂，排出第一极体。
The results indicated that nutria oocytes, like other rodent oocytes, can be matured in vitro, complete first meiotic division and discharge first polar body.
培养滴体积和培养密度显著影响卵母细胞的成熟质量和胚胎体外发育能力。
The medium volume and density affect maturation quality of oocyte and embryonic development significantly.
这表明PVP代替血清进行牛卵母细胞体外成熟培养是可行的，但是仍有待于进一步完善和加强。
This indicated that it was possible to replace serum by PVP in bovine IVM with some other improvement.
未成熟卵母细胞体外成熟培养为解决多囊卵巢综合征不孕患者生殖、生育问题提供新的治疗途径。
Oocyte retrieval after in-vitro maturation (IVM) provides a new treatment option, especially for infertile women with polycystic ovary syndrome (PCOS).
体外培养成熟的卵母细胞，吸卵管反复吹打去除颗粒细胞，电子显微镜下观察其部分超微结构。
After the completion of culture of oocytes for IVM, cumulus cells were removed. Ultrastructure was studied under electron microscope.
添加EGF，采用共培养方式成熟的卵母细胞，GSH含量高，超微结构更加健康。
There were high GSH concentration and healthy ultrastructure in EGF-added and coculture medium.
背景与目的：建立一种稳定的人卵母细胞体外培养成熟技术。
BACKGROUND AIM: To establish a stable in vitro culture technique of human oocytes.
方法：采用小鼠卵母细胞体外培养、体外受精的方法研究了铅对小鼠卵母细胞的成熟和体外受精的影响。
Methods the effects of TGA on oocyte maturation and in vitro fertilization (IVF) in mice were studied by the method in vitro culture and IVF in mice oocyte.
卵母细胞体外成熟的影响因素很多，成熟培养时间就是其中一个重要的影响因素。
There are many factors which influence the efficiency of maturation in vitro, and duration is one of the factors.
在牛卵母细胞体外成熟培养过程中，本研究在同一种成熟培养液中，分别添加用0.3％PVP和10％FBS，检验PVP代血清培养是否可行。
During the bovine oocyte maturation, this research examined the possibility of serum-free maturation which 10%FBS was replaced with 0.3%PVP.
在牛卵母细胞体外成熟培养过程中，本研究在同一种成熟培养液中，分别添加用0.3％PVP和10％FBS，检验PVP代血清培养是否可行。
During the bovine oocyte maturation, this research examined the possibility of serum-free maturation which 10%FBS was replaced with 0.3%PVP.
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