蛋白的测定采用蛋白质印迹法。
用免疫蛋白印迹法检测细胞VEGF蛋白表达。
Western blotting was used to detect protein expression of VEGF.
通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。
Sandwich ELISA, dot blotting and DNA sequencing methods were used for the identification of positive clones.
采用斑点印迹法观察黑色普氏菌能否与人血红蛋白结合。
Dot blot assay was adopted to observe the binding of hemoglobin by P. nigrescens.
利用蛋白质免疫印迹法或免疫电泳方法检查抗体产生情况。
Check the purity of eluted protein with SDS/PAGE and identify the titer of purified antibody with immune electrophoresis.
方法采用免疫组织化学技术、图像分析、放射免疫和免疫印迹法。
Methods Immunohistochemical technique, image analysis, radioimmunoassay and Western blotting were used.
蛋白质印迹法：蛋白质分子从电泳凝胶转移到固相介质，然后用抗体进行免疫检测的技术。
Western blotting --- A technique analogous to Southern blotting, used for detection of proteins, usually by immunological methods.
结果：经荧光显微镜、流式细胞仪及免疫印迹法检测，证实AIRE基因得到了稳定表达。
Results: Stable expression of AIRE was confirmed by fluorescence microscope, FACS and western blotting analysis.
方法 采用免疫组织化学染色法、免疫印迹法，分析检测食管癌标本中EZH2蛋白的表达。
We then detected EZH2 protein expression in the esophageal squamous carcinoma, paracarcinomatous and normal tissues by Western blotting.
用蛋白质免疫印迹法 （Westernblotting）测定血小板内蛋白酪氨酸磷酸化的表达。
Protein tyrosine phosphorylation in platelets was assayed by Western blotting and platelet aggregation was assayed by nephel omete r.
经生物素标记探针杂交确认RANTES基因的整合，用免疫斑点印迹法对重组基因表达水平进行定量测定。
The integration of RANTES gene was confirmed by hybridization with biotin-labelled probes and the recombinant gene expression level was quantitatively detected by immunodot blotting.
方法应用免疫组织化学方法检测erk在脊髓后角的定位表达，并用图像分析系统和免疫印迹法进行定量分析。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
新的抗体连接方法是为蛋白质印迹法专门设计的。后者是一种凝胶电泳法可根据细胞和组织样本的分子重量对其进行分离。
The new antibody conjugates are designed for Western blotting applications, which use gel electrophoresis to separate proteins from a sample of cell or tissue based on molecular weight and size.
方法：用免疫印迹法检测aif在细胞中的表达量；用免疫组化法检测aif在29例胃癌组织和10例癌旁正常胃组织中的表达。
Immunohistochemical method was used to detect the expression of AIF in 29 cases of gastric cancer tissues and 10 cases of normal gastric mucosa.
方法利用免疫细胞化学，蛋白质印迹法，RT -PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。
Methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry.
之后，他们又应用金标准试验——蛋白印迹法(Westernblot)检测了229名阳性患者，结果表明快速检测结果中有超过10%为假阳性。
They then checked 229 positive patients with the gold-standard laboratory test, a Western blot. More than 10% of the rapid tests were giving false bad news.
核酸杂交试验：是检测HPV感染的重要的手段，包括斑点印迹法（dotblothybridization）、组织原位杂交法、核酸印记法（Southern blot hybridization）。
Nucleic acid hybridization test: it is an important means to detect HPV infection, including dot blot method (blot hybridization dot), tissue in situ hybridization, blot hybridization (Southern).
目的比较酶联免疫（ELISA）、放射免疫（RIA）和免疫印迹（IB）法检测胰岛素自身抗体（IAA）敏感性和特异性的差别。
Objective To compare the difference of enzyme-linked immunosorbent assay(ELISA), Radioimmunoassay(RIA) and immunoblotting(IB) in the determination of insulin autoantibody(IAA).
方法：采用双向电泳免疫印迹检测法分析了83例CHD患者血清hdl亚类组成及相对百分含量。
Methods: The contents of serum HDL subclasses in 83 CHD subjects were determined by two dimensional gel electrophoresis associated with immunodetection method.
方法用自动分析仪测定生理代谢参数，用免疫印迹和免疫荧光法检测glut1。
Methods Physiological parameters of myocardial metabolism were measured with automated analyzers, and GLUT1 distribution was assessed by immunoblot analysis and immunofluorescence.
通过振荡吸附法对聚合物的结合特性进行了评价，发现印迹聚合物微球对模板分子的识别选择性优于块状印迹聚合物和非印迹聚合物。
Binding property was evaluated with a batch adsorption method. The results indicate that the selective recognition of the template molecule with the molecularly imprinted Po...
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
采用紫外分光光度法对印迹聚合物的吸附性能和选择性识别能力进行了研究。
The adsorption performance and selective recognition ability of MIPs was also studied by UV spectrophotometry.
采用蛋白印迹和ELISA法对表达产物进行特异性鉴定。
The specificity of the expressed products was proved by the immunoblot (WB) and ELISA.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
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