用单链构象多态性对扩增片段进行点突变检测。
The point mutations in these fragments were detected by SSCP.
方法:聚合酶链反应(PCR)、单链构象多态性(SSCP)分析。
Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.
目的:利用单链构象多态性(SSCP)对两起食物中毒的致病菌进行快速分析。
Objective: To quickly detect two pathogenic bacteria strains of food poisoning by single strand conformation polymorphism (SSCP) assay.
用PCR -单链构象多态性(SSCP)和DNA序列分析方法确定扩增序列突变情况。
Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.
方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
目的:评价异源双链分析(HET)和单链构象多态性分析(SSCP)在基因突变检测中的作用。
Objective:To assess the roles of single stranded conformation polymorphism(SSCP)and heteroduplex polymorphism(HET)in screening gene mutation.
目的:观察单链构象多态性(SSCP)筛查akt2基因突变时凝胶配制的不同对筛查结果的影响。
Objective: To observe the effect of different gel preparation on detecting mutation of AKT2 gene by single-strand conformation polymorphism (SSCP).
采用PCR单链构象多态性技术(SSCP)结合DNA序列测定证实,对病人SCN5A的全部2 8个外显子进行突变检测。
PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.
方法采用聚合酶链式反应-单链构象多态性方法检测46例高血压患者和39例正常血压对照者的胰岛素受体基因第17、18外显子的多态性。
Methods The polymorphism if exon 17 and 18 of insulin gene were detected in 46 hypertension patients and 39 normal controls using PCR and single strand conformation polymorphism.
用聚合酶链反应和单链构象多态性( PCR-S SCP)结合序列测定方法,检测了66例健康对照I L-10启动子区多态性的发生。
The Polymorphisms of IL 10 Promoter region were detected by PCR SSCP and sequencing. 66 of health control were examined by this way.
方法:应用免疫组织化学和聚合酶链反应—单链构象多态性分析(PCR-SSCP)检测10例舌白斑和32例舌癌,并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
方法:应用免疫组织化学和聚合酶链反应—单链构象多态性分析(PCR-SSCP)检测10例舌白斑和32例舌癌,并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
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