• 单链构象多态性对扩增片段进行突变检测。

    The point mutations in these fragments were detected by SSCP.

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  • 方法聚合酶链反应PCR)、单链构象多态SSCP)分析。

    Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.

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  • 目的:利用单链构象多态(SSCP)对食物中毒致病菌进行快速分析

    Objective: To quickly detect two pathogenic bacteria strains of food poisoning by single strand conformation polymorphism (SSCP) assay.

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  • PCR -单链构象多态(SSCP)DNA序列分析方法确定扩增序列突变情况。

    Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.

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  • 方法应用PCR单链构象多态(SSCP)分析技术结合基因序列测定方法确定突变类型

    Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.

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  • 目的评价异源双链分析(HET)单链构象多态分析(SSCP基因突变检测中的作用

    Objective:To assess the roles of single stranded conformation polymorphism(SSCP)and heteroduplex polymorphism(HET)in screening gene mutation.

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  • 目的观察单链构象多态(SSCP)筛查akt2基因突变时凝胶配制不同对筛查结果的影响

    Objective: To observe the effect of different gel preparation on detecting mutation of AKT2 gene by single-strand conformation polymorphism (SSCP).

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  • 采用PCR单链构象多态技术(SSCP结合DNA序列测定证实病人SCN5A全部2 8个外子进行突变检测。

    PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.

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  • 方法采用聚合酶链式反应-单链构象多态方法检测46例高血压患者39例正常血压对照者胰岛素受体基因第1718外显子的多态性。

    Methods The polymorphism if exon 17 and 18 of insulin gene were detected in 46 hypertension patients and 39 normal controls using PCR and single strand conformation polymorphism.

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  • 聚合酶链反应单链构象多态( PCR-S SCP)结合序列测定方法检测了66例健康对照I L-10启动子多态发生。

    The Polymorphisms of IL 10 Promoter region were detected by PCR SSCP and sequencing. 66 of health control were examined by this way.

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  • 方法应用免疫组织化学聚合酶链反应—单链构象多态分析(PCR-SSCP检测10白斑32癌,结合临床资料进行分析。

    Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.

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  • 方法应用免疫组织化学聚合酶链反应—单链构象多态分析(PCR-SSCP检测10白斑32癌,结合临床资料进行分析。

    Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.

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