目的:评价异源双链分析(HET)和单链构象多态性分析(SSCP)在基因突变检测中的作用。
Objective:To assess the roles of single stranded conformation polymorphism(SSCP)and heteroduplex polymorphism(HET)in screening gene mutation.
方法:应用免疫组织化学和聚合酶链反应—单链构象多态性分析(PCR-SSCP)检测10例舌白斑和32例舌癌,并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
目的:利用单链构象多态性(SSCP)对两起食物中毒的致病菌进行快速分析。
Objective: To quickly detect two pathogenic bacteria strains of food poisoning by single strand conformation polymorphism (SSCP) assay.
方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
用PCR -单链构象多态性(SSCP)和DNA序列分析方法确定扩增序列突变情况。
Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.
方法:聚合酶链反应(PCR)、单链构象多态性(SSCP)分析。
Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.
方法:聚合酶链反应(PCR)、单链构象多态性(SSCP)分析。
Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.
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