目的探讨在不同的免疫学检测方法中，与单链抗体相连的亲和标记肽对单链抗体与抗原结合活性的影响。

Objective to investigate the affects of affinity tag on binding activity of single chain variable fragment (ScFv) to antigen using different immunological assays.

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结论：成功地构建了人源抗乳腺癌单链抗体基因片断，为进一步构建人源抗乳腺癌单链抗体库提供了试验基础。

Conclusion: the human single-chain antibody gene fragments were constructed successfully, which provides a way for constructing the human single-chain antibody library of anti-breast cancer.

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结论：成功地构建了人源抗乳腺癌单链抗体基因片断，为进一步构建人源抗乳腺癌单链抗体库提供了试验基础。

Conclusion: the human single-chain antibody gene fragments were constructed successfully, which provides a way for constructing the human single-chain antibody library of anti-breast cancer.

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