目的:介绍一种简单有效的单细胞分离技术。
OBJECTIVE: To introduce a simple and effective method for single cell separation.
结果与结论:单细胞分离的成功率达到96.5%。
RESULTS AND CONCLUSION: Success rate of single cells separation was 96.5%.
悬浮培养8天左右的玫瑰茄细胞最适宜于单细胞分离;
The cell suspension cultures for 8days were most favourable for the formation of cell clones.
掌握简单有效的单细胞分离技术为准确、定量的单细胞水平研究奠定基础。
A simple and effective method for single cell separation would be a foundation of exactly and quantitative study at single cell level.
随着单细胞分离技术及单细胞测序技术日益成熟,单个肿瘤细胞全基因组测序已成为肿瘤研究的一个崭新的领域。
With the development of the technology of single-cell separation and sequence, sequencing single tumor cell has become a new area of cancer research.
ACT小组也将实验中的这些胚胎组织冻存,使用分离的单细胞作为人类胚胎干细胞的来源。
The ACT team also froze the embryos and used the single cell that was removed as a source of human embryonic stem cells.
运用单细胞凝胶电泳技术,研究了太原市大气细颗粒物(PM 2.5)对分离的大鼠肺泡巨噬细胞DNA的损伤作用。
Damage from atmospheric fine particles (PM2.5) in isolated DNA of alveolar macrophages of rats in Taiyuan City was studied applying single cell gel electrophoresis technique.
方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞;
Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
对单细胞分析化学中毛细管电泳分离技术的进样方式、检测器、应用和前景予以综述,引用文献46篇。
Injection modes, detectors and future prospects of this micro separation technique for single cell analysis are described in detail.
简单介绍了毛细管电泳在医药领域中的应用,包括药物分析、中药成分分析、手性对映体分离分析和临床化学、法医及单细胞分析等。
A review with 68 references is presented on the application and new developments of CE in pharmaceutical analysis, clinical chemistry and single cell analysis etc.
采用冷胰酶消化法分离培养大鼠睾丸支持细胞,采用单细胞凝胶电泳法检测亚硝酸钠对大鼠睾丸支持细胞dna的损伤作用。
Method: The effects of sodium fluoride on DNA damage and repair in isolated male mice testicular cells were observed with single cell gel electrophoresis test (Comet assay).
采用染色细胞分离的方法对该技术分离单细胞的效率进行验证。
Success rates of separation were evaluated by separated staining of single cells.
采用染色细胞分离的方法对该技术分离单细胞的效率进行验证。
Success rates of separation were evaluated by separated staining of single cells.
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