自从1975年发现了单克隆抗体技术和不久后提出生物导弹设想以来，导向治疗受到许多科研工作者的关注。
Since 1975 when the technology of hybridoma and monoclonal antibody was developed, many investigators have been paying close attention to antibody targeting therapy for patients with cancer.
方法应用单克隆抗体技术对32例乳腺癌患者进行了外周血T淋巴细胞亚群的测定，并与35名正常健康作对照。
Methods T lymphocyte subgroup level was determined by monoclon antibody in 32 cases of breast cancer and 35 normal controls.
方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。
Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.
利用抗体库技术可制备人源单克隆抗体。
Human monoclonal antibodies can be made by means of antibody library technology.
C6 (R)技术平台，开发了大规模生产的生物制药产品，如重组蛋白，其中包括单克隆抗体。
C6 (r) technology platform has been developed for the large-scale manufacture of biopharmaceutical products such as recombinant proteins, including monoclonal antibodies.
用杂交瘤技术获得了第二组抗人血小板的单克隆抗体。
We have obtained the second group of monoclonal antibodies against human platelet by means of hybridoma technique.
目的：建立一条从孕早期妇女血清中分离纯化早孕因子(EPF)的技术路线，获得高纯度的EPF，并对制备抗epf单克隆抗体进行阶段性的初步尝试。
Object: Set up a method of isolation and purification of early pregnancy factor (EPF) from human pregnancy sera and get pure EPF, then attempt to prepare monoclonal antibodies.
方法采用LRP单克隆抗体、流式细胞技术分别测定15例单纯缺铁性贫血患者（对照组）和65例AL患者（AL组）LRP的表达率。
The expression rate of LRP was detected by its monoclonal antibody and flow cytometry in 15 asiderotic anemia patients and 65 AL patients.
二者的结构多肽NP蛋白和F蛋白用特异性单克隆抗体免疫转印技术分析，未显示差异。
The viral NP and F proteins revealed the same patterns by Western Blotting with specific monoclonal antibodies.
应用单克隆抗体一胶体金标记技术，采用徒手 切片 法和斑点试验 法检测柑桔黄龙病获得成 功。
The research had succeeded in detecting the Citrus Huanglongbing through the hand-sliced method or dot -marked observation by the monoclonal antibody with colloidal gold -labeled (MACG) technique.
利用单克隆抗体间接竞争ELISA技术检测氟虫腈在青菜和土壤中的降解规律。
Indirect competitive ELISA based on monoclonal antibody was applied to monitor the degradation kinetics of fipronil in vegetable and soil.
利用蛋白免疫印迹及ELISA技术研究重组蛋白与小鼠抗人DR5单克隆抗体AD5-10的结合情况。
The binding ability of these deletant proteins to AD5-10, a mouse anti-human DR5 monoclonal antibody, was evaluated by immunoblotting and ELISA.
采用杂交瘤技术制备人源性单克隆抗体存在许多困难，90年代初期出现了抗体库技术，为抗体的制备提供了新的途径。
But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody.
方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
DNA改组技术是目前蛋白质、酶和单克隆抗体等体外定向进化的高效方法。
Recently DNA Shuffling has been developed to one of the most effective methods for evolutions of protein, enzyme and monoclonical antibody in vitro, etc.
方法：应用荧光标记单克隆抗体和流式细胞技术。
METHODS: Using fluorescent-labeled monoclonal antibodies and flow cytometry.
方法：应用荧光标记单克隆抗体和流式细胞技术。
METHODS: Using fluorescent-labeled monoclonal antibodies and flow cytometry.
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