试剂盒中96孔板中的包被抗原为原核表达的重组P 30蛋白，其具有良好的抗原性。
The coating antigen in a 96 pore plate in the kit is the prokaryotic expression recombinant P30 protein, and has good antigenicity.
目的：分析ELISA包被抗原在检测溃疡性结肠炎（UC）血清ACA的非特异性干扰物。
Objective: To analyze the nonspecific interferents in detecting ACA-IgG in sera of patients with UC by ELISA.
依照活性酯法将OA与OVA偶联作为包被抗原，建立了间接竞争ELISA检测OA的方法。
OA-OVA, as the coating antigen, were prepared by activated ester method, and at last we established the indirect competitive ELISA for detecting OA.
以P25基因的重组表达产物为包被抗原，建立了检测鸭疫里默氏菌血清抗体的间接ELISA。
An indirect ELISA was developed for detecting RA infection in ducks, based on P25 as the coated antigen.
通过条件试验，确定了所获抗血清和包被抗原的最佳工作浓度分别为1：6000和1：25600。
The best concentration of the antiserum and the coated antigen were fixed on 1:6000 and 1:25600 by tests on condition, respectively.
试剂盒采用原核表达的重组P30蛋白作为包被抗原，依据竞争ELISA原理检测猪血清中非洲猪瘟病毒的抗体。
The kit adopts prokaryotic expression recombinant P30 protein as coating antigen, and detects the African swine fever virus antibody in pig serum according to a competitive ELISA principle.
本试验分别使用超声波裂解抗原、脂多糖蛋白抗原和全菌抗原作为包被抗原，建立相应的检测方法并进行重复性试验。
The indirect ELISA is widely used for detection of antibodies and coating antigen is very important to the accuracy of ELISA experiment.
将表达的融合蛋白作为包被抗原，建立了间接酶联免疫吸附试验(间接elisa)的诊断方法以检测CDV血清抗体水平。
The method of indirect ELISA to detect CDV antibody in blood serum was established, using recombinant nucleocapsid protein as envelope antigen.
方法：制备SARS患者肺组织包被抗原，采用酶联免疫吸附试验(EL IS A)检测SARS患者血清中特异性抗体。
Methods: Specific antibodies were detected with enzyme linked immunosorbent assay (ELISA) using coated lung tissue as antigen and serum of SARS patients as antibodies.
用表达的口蹄疫3a蛋白作为包被抗原，建立了间接elisa试验，对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。
An indirect ELISA was established with expressed 3a protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera, immune sera and other disease sera.
将表达的重组E2蛋白纯化后作为ELISA包被抗原，建立了一种基于重组E2蛋白的间接ELISA方法，并对此方法的反应条件进行了优化。
An indirect ELISA was developed and optimized with the purified recombinant E2 protein as coating antigen. The optimal conditions of ELISA were determined as follows.
利用重氮化两步法将盐酸克伦·特罗(CL)分别偶联到牛血清蛋白(BSA)和卵清蛋白(OVA)上制得了免疫抗原bsa - CL和包被抗原ova - CL。
Conjugates of clenbuterol hydrochloride (cl) to protein bovine serum albumin (BSA) and ovalbumin (ova) were prepared via diazo coupling to form immunogen BSA-CL and OVA-CL.
用卵清白蛋白与伏马菌素B2的偶联物（OVA-FB2）做包被抗原，标准伏马菌素B2（FB2）做竞争抗原，初步建立了检测玉米粉中伏马菌素B2的间接竞争ELISA方法。
An ic-ELISA for detecting fumonisin B2(FB2) of maizena was developed by using the coating antigen of OVA-FB2 and competitive antigen of FB2.
用卵清白蛋白与伏马菌素B2的偶联物（OVA-FB2）做包被抗原，标准伏马菌素B2（FB2）做竞争抗原，初步建立了检测玉米粉中伏马菌素B2的间接竞争ELISA方法。
An ic-ELISA for detecting fumonisin B2(FB2) of maizena was developed by using the coating antigen of OVA-FB2 and competitive antigen of FB2.
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