取大鼠肱三头肌,采用二步消化法分离骨骼肌卫星细胞,体外进行原代培养和传代培养。
Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
该算法以层次包围盒(obb)为基础,通过为每个骨骼模型建立包围盒,运用分离轴理论计算包围盒的相交测试。
Based on OBB (oriented bounding box), this algorithm constructs an OBB for each bone model and computes intersect tests of the OBB with separation axis theory.
目的探讨骨骼肌卫星细胞的分离、培养及鉴定方法。
Objective to establish the methods for culture and identification of skeleton satellite cell.
日本的调查人员从牛奶中分离出一种叫做激肽原的蛋白质,并用实验室实验证明了它确实能促进骨骼生长。
Japanese investigators isolated a protein, kininogen, from milk and demonstrated in laboratory experiments that it promotes bone growth.
巴西的研究小组发现,在活体中人类输卵管间质干细胞能轻易的分离和扩展,能分化成肌肉、脂肪、软骨和骨骼细胞株。
The Brazilian team found that human fallopian tube mesenchymal stem cells could be easily isolated and expanded in vitro, and are able to differentiate into muscle, fat, cartilage and bone cell lines.
目的完善骨骼肌细胞的分离培养方法,为有关实验提供足量的细胞。
Objective To improve the method of isolated culture of mice skeletal muscle cells and supply enough cells for some experiments concerned.
方法:1体外分离培养大鼠骨骼肌卫星细胞,免疫组化鉴定骨骼肌卫星细胞并检测其纯度。
Methods: 1. Primary rat skeletal muscle satellite cell was cultivated in vitro. The cultured cells were identified with cellular immunochemical stain.
干预:分离新生小鼠骨骼肌卫星细胞,进行体外培养。
INTERVENTIONS: the skeletal muscular satellite cells of the neonatal mice were isolated and cultured in vitro.
干预:分离新生小鼠骨骼肌卫星细胞,进行体外培养。
INTERVENTIONS: the skeletal muscular satellite cells of the neonatal mice were isolated and cultured in vitro.
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