方法：分离单个核细胞。
方法为血液采集后，离心分离单个核细胞，进行冷冻保存及复苏后洗涤。
The mononuclear cell are separated from blood after blood collection, then cryopreserved and washed after thawed.
目的：从脐带血中分离单个核细胞以获得血管内皮前期细胞，并观察其生物学行为。
Objective to isolate endothelial progenitor cells from umbilical cord blood and observe their biologic behaviors.
同时由于脐血库液氮保存设备费用昂贵，需要预先分离脐血单个核细胞以减少样本冻存体积，以满足建库实际需求。
Cord blood separation and volume reduction is first needed to meet THD practical bank requirement for the high expense of liquid nitrogen preservation equipment.
感染后第10、35天分离外周血单个核细胞用于抗原特异细胞增殖实验；
Peripheral blood mononuclear cells were collected at 10,35 days postinoculation(p. i) and used to conduct antigen specific blastogenesis assay.
目的：探索一种简便、高效的脐血单个核细胞分离方法。
Objective: To explore an convenient and high-performance isolation method of mononuclear cells derived from human cord blood.
本文旨在比较差速贴壁方法分离的小鼠骨髓单个核细胞诱导分化为内皮祖细胞的生物学特性。
The different biological functions were studied in mouse bone marrow-derived endothelial progenitor cells isolated by differential time attachment.
无菌条件下取正常足月剖宫产胎儿的脐带血，经肝素抗凝，用淋巴细胞分离液分离脐血单个核细胞。
The specimens were obtained sterilely with preservative-free heparin, and the cord blood mononuclear cell was isolated by lymphocyte separation medium.
血细胞分离机可以安全、有效地应用于肿瘤患者以生物治疗为目的的外周血单个核细胞的采集。
The blood cell separator can be safely and effectively used in acquisition of mononuclear cells in peripheral blood for the purpose of biotherapy in cancer patients.
血细胞分离机可以安全、有效地应用于肿瘤患者以生物治疗为目的的外周血单个核细胞的采集。
The blood cell separator can be safely and effectively used in acquisition of mononuclear cells in peripheral blood for the purpose of biotherapy in cancer patients.
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