将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上，构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
目的构建结核杆菌分泌蛋白mpt53原核表达载体并进行表达和纯化。
Objective to construct a expression vector of MPT53 of mycobacterium tuberculosis and identify and purify the protein in the e.
外源淀粉水解酶能在酵母中高效表达并分泌，其主要因素在于：在构建载体时组入强启动子；
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
目的：克隆人和小鼠的活化t细胞表达与分泌调节基因(RANTES基因)并分别构建腺病毒表达载体。
Objective: to clone the human and murine origin RANTES genes and construct the adenoviral expression vectors.
本文将从信号肽结构特征、常用的信号肽序列以及构建高分泌型载体等方面进行综述。
This article reviews signal peptide sequence about its structural feature, the common used signal peptide and the construction of vector with high secretory.
结论构建了人瘦素的哺乳动物细胞表达载体，并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
结论构建了人瘦素的哺乳动物细胞表达载体，并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
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