结果：该杂交瘤细胞株分泌抗人脑胶质瘤细胞单克隆抗体H12具有与脑胶质瘤细胞特异性结合的特性。
Result:The monoclonal antibody H12 against human glioma which the hybridizing cell secretions can consociate specifically with human glioma.
目的建立分泌抗华支睾吸虫代谢抗原的单克隆抗体杂交瘤细胞株。
Then the hybridoma cell lines secreting high titer of McAbs were screened.
研究者希望能设计一种治疗性的疫苗，减缓HIV的疾病进程，使失效的B细胞恢复分泌高质量抗体的能力。
The researchers hope to design a therapeutic vaccine, slow HIV disease process, so that failure of B-cell secretion restore the ability of high-quality antibodies.
方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
这类B细胞表现出早熟和低抗hiv的能力下降，分泌抗体的能力下降。
B cells showed such precocious and low anti-HIV decline in the ability, the ability of antibody production decline.
结论：杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体，利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
结论：杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体，利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
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