方法用淋巴细胞分层法和低渗溶血法提取PBMC。
Methods Peripheral blood mononuclear cells(PBMC) were collected by using lymphocyte Ficoll and the low-osmotic hemolysis method.
采用液体双相分层法和等电点沉淀法提取苏云金芽孢杆菌伴胞晶体蛋白。
It is reported that a comparison with the different of extracting parasporal Crystal Protein of Bacillus thuringiensis between the liquid double-phase method and Isoelectric point method.
采用底部剪力法和分层法对框架竖向、横向水平荷载作用下的内力和侧移进行计算。
The bottom shear method and the method of vertical, layered framework lateral loads of internal force and lateral to calculate.
水平荷载作用下内力的计算用替代刚架法，竖直荷载作用下内力的计算可用弯矩分配法或分层法。
Hand calculation includes substitute rigid frame method under horizontal loads and moment distribution or decomposed storey method under vertical loads.
介绍了净化再生酸的静置分层法，将处理剂anpa加入再生酸中，搅拌后静置，使再生酸分层分离而得到净化。
A standing and layering process for the regenerated acid purification is described. The treating agent ANPA is added in regenerated acid and stood after agitation to purify it through layering.
在印度旁遮普省昌迪加尔的45所托儿所(anganwadi)中心采用多级分层随机取样法选择了806名6岁以下儿童。
We randomly selected 806 children under 6 years of age from 45 primary anganwadi (childcare) centres in Chandigarh, Punjab, India, that were chosen through multistage stratified random sampling.
可以使用分类法或其他一些方法(比如按日期范围编组，如示例中所示)以分层的方式编组这些值。
The values can be organized hierarchically in a taxonomy or in some other way, such as grouping dates into ranges, as in the example.
群桩中桩身压缩由单桩静载试验得到的桩顶沉降与桩端沉降之差求得，群桩的桩端沉降由分层总和法计算。
The compression of the pile in the pile group can be determined by static load test. The settlement of the pile toe is calculated by layer wise summation method.
介绍了用分层装药法拆除车间内钢筋混凝土基础的设计、施工，此工程的设计参数和施工方法对类似工程有一定的参考价值。
The design and implementation are introduced to demolish two reinforced concrete foundations by stratification loading explosive in work shop. A reference is available for similar engineering.
调查在分层逐级抽样法与随机抽样法相结合的抽样基础完成，共收集有效样本1005个。
The survey was completed on the basis of combining stratified sampling and random sampling, collecting 1005 valid samples in total.
因此国外厂家对超高压交联聚乙烯电缆如果采用“挤塑模铸法”，目前均采取“分层次挤塑硫化”工艺方式。
Therefore, if the foreign plants adopt plastic extrusion molding for over-voltage crosslinked polythene cable, they currently adopt "plastic extrusion sulfuration by layers" method.
采用双曲线和指数曲线两种拟合方法对实测数据进行了曲线拟合计算，同时采用分层总和法对路基总沉降进行了计算。
The hyperbolic curve method and the exponential curve method are used to forecast embankment settlement of the highway around Harbin city, meanwhile total embankment settlement is calculated.
采用多阶段分层随机抽样法对2岁以下儿童进行疾病状况询问调查。
Multistage stratified random sampling was conducted an interview survey in status of illness for children under 2 years old.
若媒质分层均匀，则经典镜像法需要无穷多个镜像来模拟这种电场问题，这给分析计算带来一定的困难。
However, an infinite number of images must be employed in the calculation of the field with multi layer media, which results in many difficulties.
基于弹性理论方法，结合分层总和法，编制了群桩沉降计算程序，并掘此对普通群桩和组合桩群桩性状和沉降计算进行研究。
Based on elastic methods and the layer-wise summation method, a program developed for settlement calculation of pile ground and combined composite foundation.
本文根据分层总和法的基本概念及表达式给出了变形系数表及变形梯度系数表。
Based on the fundamental concept of layer-wise summation method, this paper presents tables for deciding the coefficients of deformation and deformation grade.
对于群桩，将群桩视为实体基础，按分层总和法计算，但未给出相应的限值。
For pile group, the settlement can been calculated by layer and summation mouth by pile group is considered body foundation, but have not given the limits value.
根据群落的垂直结构和成层现象，应用红外线CO_2气体分析法，分层测定了主要植物22种58株的光合速率。
The photosynthetic rates of 58 individuals of 22 main species at different layers of community were measured with an infrared CO_2 analyzer.
本研究利用边界元法求解了悬板分层式引水渠首的悬板区过流问题。
Boundary element method to calculate the free surface flow over the plate region of the hanged-plate two storied intake work is presented in this paper.
但是，出于针刺安全和分层次刺法的要求，腧穴的定位描述中“解剖”一项依然保存了下来。
However, the anatomical representation of acupoints was preserved because of the requirement for safety and the layered needling method.
先房后柱的上向分层胶结充填法在我国应用较为广泛，但矿柱后期回采条件较差。
The practice cf mining room first and then pillars by overhand cut and cemented fill is widely used in China, but the condition for pillars recovery in later period is normally worse.
地基的沉降变形依据不同地基土的压缩模量，采用分层总和法来确定。
And the foundation settlement is calculated by soil compression modulus, using layer dividing summation method.
采取分层抽样法，从青岛某理工院校抽取被试850名，收归有效问卷809份，有效问卷归收率为95.18%。
Stratified sampling method, from a polytechnic school in Qingdao test samples were 850, 809 valid questionnaires were valid for the 95.18 percent response rate.
对于金属矿的开采，一般认为分层崩落法与其他采矿法相比，是一种成本高、产量低、木材消耗量大的采矿方法。
Generally top slicing is a mining method of high cost, low productivity, and large consumption of timber as compared with other methods for mining metalliferous ore bodies.
方法采用分层随机取样法对抽取的15名陆军军人进行结构式访谈。
Methods The 15 soldiers selected through a hierarchical sampling method were treated by an un-structured interview.
利用分层分类法把厦门岛城市土地类型分为七类，并对分类结果进行了验证。
The urban land of Xiamen Island is classified into seven categories using the hierarchical classification method, and the classification results are verified.
下向分层胶结充填采矿法是矿岩破碎、开采技术条件复杂的难采矿体最有效的采矿方法。
The underhand slice stoping with cemented filling is the most effective mining method for incompetant orebody with complicated mining conditions.
结果表明，放顶煤采煤法其支承压力分布形式与分层开采法类似，但在量值及影响范围上有增减。
The result indicated That the abutment pressure distribution form was similar to that of slicing mining method but its magnitude and affecting range changed.
在分析中，上部结构用空间子结构法分析，而分层土用分层总和法求其刚度。
The space substructure method is used to analyse the space frame, and the finite layer summation method is used for the layered soil.
推荐使用机械化上向水平分层充填采矿法，提出了安全回采措施。
The upward horizontal cut and fill stoping method and the measures for safe mining are recommended.
应用推荐