结论P CR扩增法可以获得优良的DNA分子量标准。
方法运用PCR技术分别扩增不同大小的特异性条带，确定其浓度，按比例混合后组成所需要的DNA分子量标准。
Methods were amplified PCR technology for different sizes of the specificity of the band, its concentration, in proportion to the needs of mixed composition of the DNA marker.
采用蛋白质分子量标准物和纯化的重组蛋白质进行半干式蛋白质印迹，探讨半干式电泳印迹的多种因素对印迹效果的影响。
To elucidate the effect of the semi-dry electrophoretic transfer and its influencing factors in Western blot, the protein marker and purified recombination protein were used to analyzed.
鉴别和检查指标标准：通过理化性质、紫外光谱、分子量指标、指纹图谱、水解氨基酸指标五部分进行确定。
The standard of identification: it has been identified according to physiochemistry, UV spectrum, molecular weight, Chromatographic Fingerprints and amino acid analysis.
用甘油基作标准求出了聚醚的数均分子量，结果表明此法准确、方便、快速。
The glyceryl was used as standard to calculate Number—Average Molecular Weight of polyether. The result showed that it was accurate, convenient and high speed method.
实验结果表明，利用质心法测定的蛋白分子量比最大灰度法测定的更接近于标准分子量。
The experimental results show the centroid method has an advantage over the maximum gray level method.
建立反应混合物转化率与凝胶渗透色谱（GPC）谱图峰面积的标准曲线，采用GPC测定反应混合物转化率和聚合物的分子量及分布。
The thesis includes the following aspects:Based on the calibration curve established, the conversion data of reaction mixtures were determined by Gel Permeation Chromatography (GPC) method.
建立反应混合物转化率与凝胶渗透色谱（GPC）谱图峰面积的标准曲线，采用GPC测定反应混合物转化率和聚合物的分子量及分布。
The thesis includes the following aspects:Based on the calibration curve established, the conversion data of reaction mixtures were determined by Gel Permeation Chromatography (GPC) method.
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