鹿茸细胞采用常规细胞DMSO冻存法复苏贴壁率优于程序式冻胚仪冷冻法、乙二醇常规细胞冻存法。
The antler cell adopt cell routine DMSO frozen methods is superior to program frozen methods and ethylene glycol cell routine freeze methods.
以聚偏氟乙烯(PVDF)为第二组分聚合物与三醋酸纤维素(CTA)共混，通过冻胶法纺丝工艺制备成中空纤维纳滤膜。
Hollow fiber NF membrane was prepared with blending of PVDF as the second component polymer and CTA, and by spinning process with gelation method.
重组人脑源性神经营养因子（BDNF）在大肠杆菌中表达，以冻干形式提供。反相高效液相色谱法和SDS-PAGE测定其纯度大于96％。
Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reverse phase-HPLC and SDS-PAGE.
为了提高大米蛋白质的提取率，研究采用冻融及低频超声波处理大米浆体后，再用碱液法提取大米中的蛋白质。
In order to enhance the rate of protein from rice, freeze-thaw and low-frequency ultrasonic methods were used before alkaline solvent was used to extract protein from rice.
因而证明，液氮冻存法是培养细胞长期保种的一个行之有效的方法。
Liquid nitrogen was shown to be an effective measure for long-term preservation of cultured hepatoma cells.
结论：该法用于水样和健康人体的冻干血浆中铜和钴的同时测定，结果满意。
Conclusions: The present method has been used to direct determine copper and cobalt in water and in plasma of human body with satisfactory results.
目的比较酸洗涤法和冻融法提取HL60细胞抗原多肽的质量。
Objective To compare the qualities of HL60 cell antigen peptides extracted with acid elution or freeze thaw method.
结果表明：一般情况下，材料与冰的法向冻粘系数大于切向冻粘系数；
The results showed that the freezing adhesive strengths between metal and ice are stronger than that non-metal to ice.
应用反复冻融法以衣藻原生质体为材料制备衣藻染色质，应用显微操作技术准确将衣藻染色质转移到烟草叶片外植体中，进行连续培养并镜检观察。
Then we exactly transformed the chromatin to the explants of the tobacco leaves by the micromanipulation, and then we cultured the explants, observed by the fluorescent microscope every day.
采用酸溶碱沉法对三批来源不同的北豆根药材分别进行提取分离，所得有效部位采用冻干技术制成粉针剂。
The effective position are extracted and then they are prepared into powder injection by the technology of freezing.
探讨了冻融法的作用机制。
The mechanism of freezing and thawing method was also discussed.
方法采用薄膜分散法和冻干工艺进行木犀草素脂质体制备，并以载药量、包封率为评价指标，优化制备处方和工艺。
Optimization of preparation and formulation was accomplished by evaluating the drug-loading amounts and the entrapment efficiency of luteolin liposomes.
采用快速冻融法研究了养护制度、轻集料的预湿程度和体积砂率对高强轻集料混凝土抗冻性能的影响。结果表明：轻集料混凝土具有良好的抗冻性能。
The effect of curing regime, pre-wetting degree of lightweight aggregate, and the volume rate of sand on the frost resistance of high strength lightweight concrete is investigated.
认为地表冻胀量是冻胀率沿整个冻深的叠加 ； 法向冻胀力则是在冻结锋面上冻胀应力沿基础影响范围之内的积分 。
The normal frost heave force is an integral of soil frost heave stress at the freezing front along the range effected by a foundation.
结论二次乳化法和冻融法结合能显著提高5-氟尿嘧啶脂质体的包封率。
Conclusion Double emulsification and freeze-thawing method can significantly increase the encapsulation efficiency 5-FU in liposomes.
应用裂解气相色谱法(PGC)分析了五株流感病毒和一株新城疫病毒感染的鸡胚尿囊冻干样品。
Five strain influenza and one strain Newcastle disease virus were analysed by pyrolysis gas chromatography (PGC).
程序降温法：以甘油和二甲基亚砜为低温保护剂，分别以不同的比例添加到含有细胞悬液的冻存管中。
Programmed cooling method: The glycerol and Dimethylsulfoxide, as cryoprotective agents, were mixed with COC1 cells suspensions at different concentration, respectively.
介绍了采用同位素稀释质谱 法（IDMS）测定 冻干人尿样品中镍、铜、铅的含量。
The method of determining Ni, Cu and Pbi n urine with isotope dilution mass spectrometry(IDMS)is described.
分别采用冻融法、逆相蒸发法、超声-匀浆-冷冻干燥法制备丹参酮IIA纳米脂质体。
Methods: TotanshinoneIIA Nano-liposomes were prepared by freeze-melt, reverse phase evaporation and ultrasonic bath-refining-freeze-drying.
冷冻冻干后，对支架材料进行组织学及扫描电镜观察，测定支架孔径和孔隙率、吸水率，并采用MTT法分析支架浸提液毒性。
The scaffolds were investigated by histological staining, SEM observation and porosity measurement, water absorption rate analysis. MTT test was also done to assess cytotoxicity of the scaffolds.
声速变化比质量损失变化明显，说明超声波法是一种检测混凝土冻融损伤的简单可靠的方法。
The variation of sound velocity was more obvious than quality loss. It was concluded that ultrasonic techno…
声速变化比质量损失变化明显，说明超声波法是一种检测混凝土冻融损伤的简单可靠的方法。
The variation of sound velocity was more obvious than quality loss. It was concluded that ultrasonic techno…
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