然后麻醉状态下无菌留取下肢骨骼肌组织标本，置消毒的冻存管中，液氮保存待测。
Skeletal muscle of hind legs was collected in sterile tube and stored in liquid nitrogen.
程序降温法：以甘油和二甲基亚砜为低温保护剂，分别以不同的比例添加到含有细胞悬液的冻存管中。
Programmed cooling method: The glycerol and Dimethylsulfoxide, as cryoprotective agents, were mixed with COC1 cells suspensions at different concentration, respectively.
程序降温法：以甘油和二甲基亚砜为低温保护剂，分别以不同的比例添加到含有细胞悬液的冻存管中。
Programmed cooling method: The glycerol and Dimethylsulfoxide, as cryoprotective agents, were mixed with COC1 cells suspensions at different concentration, respectively.
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