目的探讨不同冻存方法对脐血造血细胞的影响。
Objective To show the effects of different methods on preservation of cord blood hematopoietic cells below zero degree.
目的：了解不同冻存方法对组织工程骨生物活性的影响。
Objective: To study the effects of various methods of cryopreservation on the bioactivity of tissue engineered bone.
目前，脐血造血干细胞的低温保存主要采用的是慢速冻存方法，但是这种方法的冻存效果以及冻存技术还有需要提高和完善的地方。
At present, slow cooling is the adopted method for long-term storage for HSCs but as a conventional method, it has many disadvantages.
目的研究骺板软骨细胞冻存的方法。
Objective To study frozen reservation method of epiphyseal plate chondrocyte.
目的探讨提高大鼠胰岛冻存质量的方法，以期为建立胰岛库奠定基础。
Objective to investigate the method to improve the quality of rat islets after cryopreservation to make a basis for establishment of the islet bank.
目的建立兔角膜缘上皮细胞体外培养、冻存和复苏的方法。
Objective to establish a method of cultivating limbal corneal epithelial cells of rabbit in vitro and to study its cryopreservation and resuscitation.
方法：1。标本获取及分组采集107例白血病、20例MDS病人及20例正常对照组骨髓进行染色体培养、制备及细胞冻存。
Methods 1. Patient samples: Bone marrow or blood cells were obtained from 107 patients with leukemia, 20 patients with MDS and 20 controls for chromosome and frozen cell purpose.
因而证明，液氮冻存法是培养细胞长期保种的一个行之有效的方法。
Liquid nitrogen was shown to be an effective measure for long-term preservation of cultured hepatoma cells.
采用机械方法传代，常规方法冻存和复苏。
The cells were passaged by mechanical methods, frozen and thawed by general procedure.
方法采用体外细胞培养技术进行皮肤成纤维细胞的分离、培养、传代、冻存、复苏及鉴定。
Methods Cell culture in vitro technique was used, including isolation, culture, subculture, frozen storage, recovery and identification of human fibroblasts, in this study.
关节软骨也可用类似方法冻存。
Articular cartilage also can be cryopreserved in this manner.
摘要：唾液多肽组学为疾病相关的生物标记物研究提供了新方法，但冻存条件对分析结果的影响并不清楚。
Abstract: the salivary peptidomics provides a new method for study of disease biomarkers, but the effect of frozen storage conditions on analysis results is not very clear.
卵巢组织冻存已作为恢复卵巢功能早衰及保存年轻肿瘤患者卵巢功能的方法之一进行研究。
Ovarian tissue cryopreservation is being developed as a method for preserving the ovarian function of young women at risk of early ovarian failure and young cancer patients.
对E2高水平的患者采用全胚胎冻存的方法能够获得满意的妊娠结局。
Patients with high serum E2 mass concentrations on the day of hCG administration treated with all-embryo cryopreservation may result in favorable pregnancy outcomes.
对E2高水平的患者采用全胚胎冻存的方法能够获得满意的妊娠结局。
Patients with high serum E2 mass concentrations on the day of hCG administration treated with all-embryo cryopreservation may result in favorable pregnancy outcomes.
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