结论：该方法提取冬凌草叶效果较好。
综合分析评价各冬凌草样品间的差异。
And the data of different samples was compared and analysed.
目的建立冬凌草甲素的提取和分离新工艺。
OBJECTIVE To establish a new method for the extraction and separation of oridonin.
柱色谱进一步分离可制备高纯度冬凌草甲素。
Oridonin was purified by the further separation of column chromatography.
目的确定冬凌草中冬凌草甲素的最佳提取工艺。
ObjectiveTo determine the optimal extracting process of oridonin.
目的为鉴别和进一步研究开发冬凌草提供方法依据。
ObjectiveTo provide basis for the identification and the further research of R. rubescens.
本发明涉及营养及保健的一种冬凌草茶的炒制方法。
The invention relates a method for stir-frying rabdosia rubescens tea in the field of nutrition healthcare.
冬凌草中的许多成分具有抗肿瘤、抗突变、抗氧化活性。
There are many ingredients with antitumor, resistance mutations and antioxidant activity.
目的探讨冬凌草甲素对哺乳动物细胞DNA损伤的抑制作用。
Objective To study the inhibition of Oridonin on DNA damage in the mammalian cells.
结论冬凌草甲素可以制成硬脂酸纳米粒，各项物理指标稳定。
CONCLUSION Oridonin loaded stearic acid solid lipid nanoparticles were produced with stable physical properties.
结论冬凌草甲素具有明显的抑制哺乳动物细胞DNA损伤的作用。
Conclusion Oridonin has significant inhibition on DNA damage in the mammalian cells.
目的：研究冬凌草甲素诱导白血病k5 6 2细胞凋亡的机制。
Objective: to study the mechanism of apoptosis induced by Orindonin in K562 cells.
方法利用液相色谱分离技术和重结晶方法获得冬凌草中的有效成分。
Methods Liquid chromatography and recrystallization were used to obtain the monomer of rubescensin A.
结论：冬凌草甲素可能通过线粒体途径诱导PC - 3细胞凋亡。
Conclusion the apoptosis of PC-3 cells induced by oridonin might be associated with the mitochondrial pathway.
结果：不同产地、不同变异类型冬凌草中各种成分的含量有明显差异。
Result: The content of chemical compositions in Rabdosiae Rubescentis Herba from different place and different variance-types has a significant difference.
透射电镜观察冬凌草甲素作用后BGC-823细胞超微结构的改变；
The ultrastructure changes of BGC-823 cells were observed with transmission electron microscope.
目的：分离冬凌草中的水溶性成分和测定某些无机元素和氨基酸的含量。
Aim: To separate water soluble components and determine some inorganic elements and amino acids contents.
目的：测定河南栾川老君山冬凌草、毛叶香茶菜、显脉香茶菜中的氨基酸含量。
Objective: To determine amino acid in Herba Rabdosiae, Isodon Japonica Hara and Isodon Nervosus Kudo from Laojun Mountain in Luanchuan, Henan province.
本发明属于医药技术领域，提供一种冬凌草甲素聚合物胶束给药制剂及制备方法。
The invention belongs to the medicine technology field, which provides an oridonin a polymer micelle drug delivery preparation and a preparation method thereof.
方法利用光镜及流式细胞仪观察不同浓度冬凌草甲素诱导结肠癌hct8细胞凋亡的作用。
Methods apoptosis and morphology variations were investigated by microscopic inspection and flow cytometry (FCM). Results Oridonin can induce HCT8 cell apoptosis.
目的优化冬凌草甲素聚乳酸纳米粒的制备工艺，并对其理化性质和体外释药特性进行考察。
OBJECTIVE To optimize the preparation method of the oridonin-loaded polylactic-acid nanoparticles, and investigate its properties and release behavior in vitro.
冬凌草甲素是从冬凌草等植物叶子中提取出的一种贝壳杉烯二萜类化合物，具有好的抗肿瘤活性。
Oridonin anent-kaurene diterpenoid compound mainly isolated from the leaves of R. rubescen. Oridonin has been proved to possess good antitumor activity .
方法以冬凌草甲素提取率为考察指标，考查了提取方法、提取时间、提取次数对提取效果的影响。
MethodsThe orthogonal experiment was used to determine the extracting method, extracting time and extracting times with extracting yield as markers.
目的探讨冬凌草甲素体外对肝癌BEL- 7402细胞生长抑制作用及诱导细胞凋亡作用机制。
Objective to investigate the mechanism of cell growth inhibition and apoptosis inducement of oridonin on human hepatocellular carcinoma BEL-7402 cells.
结果：冬凌草甲素可显著降低K5 6 2细胞的端粒酶活性，抑制细胞的生长，诱导细胞发生凋亡。
Results: Oridonin could decrease the activity of telomerase, as well as cause apoptosis and inhibit the growth of K562 cells significantly.
本文用MNDO方法在VAX8350计算机上对冬凌草甲素及其乙酰衍生物进行了量子化学计算研究。
Study on the quantum chemical calculations is performed by means of MNDO method on VAX8350 computer for rubescensine A and its acetylate derivative.
目的：研究冬凌草甲素诱导人组织淋巴瘤u 937细胞凋亡的机制及ERK激酶在凋亡过程中的作用。
Objective: to study the mechanisms of oridonin-induced U937 cell apoptosis, and to examine the role of ERK MAPK.
结论采用微波萃取技术提取冬凌草甲素，具有提取快速、操作简便、节省溶剂和有效成分提取率高等优点。
Conclusion microwave technology for oridonin extraction has advantages of rapid extraction, simple operation, saving solvent and higher extraction yield, etc.
结果RP - HPLC法测定乙醚萃取的血浆中冬凌草甲素保留时间稳定，标准曲线线性良好，结果准确、可靠。
Results To determine the Oridonin extracted from diethyl ether of plasma by RP-HPLC method, the retention time was stable, the linear curve fitted well, and the results were accurate and reliable.
方法：采用溶剂浸渍方法提取冬凌草中的挥发性成分，用GC -MS方法对冬凌草挥发油的化学成分进行分析。
Methods: Volatile oil was separated by solvent extraction and analysed by gas chromatography coupled with electron impact ionization-mass spectrometry (GC-MS).
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