白菜遗传转化技术中最常用的方法是农杆菌介导法。
The common method of Genetic Transformation of Brassica campestris L. is Agrobacterium-mediated method.
用农杆菌介导法，成功地将番茄铁转运蛋白基因导入了苹果砧木八棱海棠。
The tomato iron transporter gene (LeIRT2) was introduced to Malus robusta Rehd. via Agrobacterium-mediated transformation to produce iron-deficiency tolerant apple rootstock.
应用花生胚小叶再生体系，以农杆菌介导法对质粒上的基因转化进行初步探讨。
Applying to leaflet regeneration system of peanut, genetic transformation of peanut using Agrobacterium-mediated approach were carrying on the research.
目前外源基因导入玉米受体细胞的方法很多，最受瞩目的是农杆菌介导法和基因枪法。
Agrobacterium and gene gun have aroused most attention among various genetic transformation methods for exogenous gene into maize.
目的：通过农杆菌介导法将白藜芦醇合酶基因转化蔓茎堇菜，并对转化条件进行优化。
Objective: The optimization of the conditions on resveratrol synthase gene transformation for Viola diffusa Ging.
目前，农杆菌介导法是苹果上应用的主要转化方法，叶片再生不定芽途径是被广泛应用的受体系统。
At present, Agrobacterium mediated transformation of leaves are widely adopted in apples, and transgenic shoots were mainly obtained from adventitious bud regeneration.
将其与GUS报告基因融合在一起，构建了植物表达载体，并由农杆菌介导法导入水稻品种‘中花11’中。
It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11, a rice variety, by Agrobacterium meditation.
优化农杆菌介导法转化棉花的主要技术参数，拓宽了棉花基因型范围，建立了四个棉花品种的高效遗传转化体系。
The main technical parameters were improved, the genotypes of cotton acceptors were widened, and a high efficient system of transformation of four varieties was established.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥，筛选抗性转基因拟南芥植株，进行PCR检测和GUS染色。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
目前用于油菜基因转化的方法有根癌农杆菌介导法、基因枪法、PEG法、激光微束穿刺法、显微注射法和花粉介导法等。
At present, there are several methods of transformation in rape, including tumefaciens-medidated genetic etc, particle gun, PEG untake, vialaser microbeam puncture and microinjection.
通过农杆菌介导，采用叶盘法将豌豆凝集素基因转化单倍体烟草，获得了转基因再生植株。
The pea lectin gene was transformed into the haploid tobacco via Agrobacterium mediation using leaf disc method and the regenerated tobacco plants were obtained.
用农杆菌介导的叶盘法将HAL1基因转入模式植物烟草，对转基因烟草的耐盐性进行评价；
The HAL1 gene was transformed into tobacco by Agrobacterium through leaf disc transformation and the salt tolerance character of transgenic tobacco was analysised.
用农杆菌介导的叶盘转化法将一个来自棉花的谷胱甘肽s转移酶(GST)基因转化到烟草中。
A glutathione S-transferase (GST) gene from cotton was introduced into tobacco by Agrobacturium-mediated leaf disk transformation method.
以农杆菌介导的叶圆盘法将HAL1基因转化烟草植株。
HAL1 gene was transformed into tobacco plants and the screened transgenic plants showed improved salt tolerance than the control plants.
以农杆菌介导的叶圆盘法将HAL1基因转化烟草植株。
HAL1 gene was transformed into tobacco plants and the screened transgenic plants showed improved salt tolerance than the control plants.
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