农杆菌转化系统是一种优良的转化系统。
Agrobacterium transformation system is an excellent transformation system.
然后将此质粒转化入农杆菌lba4404菌株。
The plasmid was then transferred into Agrobacterium strain LBA4404.
利用农杆菌注射法对草莓果实细胞进行了转化研究。
Fruit cell transformation of strawberry was carried out with injection of Agrobacterium.
白菜遗传转化技术中最常用的方法是农杆菌介导法。
The common method of Genetic Transformation of Brassica campestris L. is Agrobacterium-mediated method.
以农杆菌介导的叶圆盘法将HAL1基因转化烟草植株。
HAL1 gene was transformed into tobacco plants and the screened transgenic plants showed improved salt tolerance than the control plants.
本文综述了发根农杆菌诱导植物毛状根的方法及其分子机制。
The method of hairy root induction of plants as well as their molecular biology are reviewed.
本文研究了茶叶儿茶素对发根农杆菌的抑制作用并进行了抗酚菌株的筛选。
This paper investigated inhibition of Agrobacterium rhizogenes by tea catechin and screening of the anti-polyphenol strain.
本发明方法改良了外植体愈伤和生芽的诱导，提高了农杆菌介导的能力。
The present invention improves the callus of inducing and the bud inducing of the explants, and enhances the agrobacterium-mediated ability.
由于白菜的再生能力较低，因此农杆菌介导白菜遗传转化的研究报道较少。
The reports of this method in Brassica campestris L. can't often be seen because of its low shoot regeneration ability.
农杆菌介导的瞬时表达体系是一个研究整株植物基因沉默的快速实用系统。
The Agrobacterium-mediated transient expression analysis in intact tissues has emerged as a rapid and useful method to study gene silencing in plants.
用农杆菌介导法，成功地将番茄铁转运蛋白基因导入了苹果砧木八棱海棠。
The tomato iron transporter gene (LeIRT2) was introduced to Malus robusta Rehd. via Agrobacterium-mediated transformation to produce iron-deficiency tolerant apple rootstock.
因此，在农杆菌介导高羊茅遗传转化中，抑菌剂以选用羧苄青霉素较为合适。
Therefore, carbenicillin was the preferable choice of the two tested antibiotics for Agrobacterium tumefaciens-mediated transformation of tall fescue.
应用花生胚小叶再生体系，以农杆菌介导法对质粒上的基因转化进行初步探讨。
Applying to leaflet regeneration system of peanut, genetic transformation of peanut using Agrobacterium-mediated approach were carrying on the research.
介绍农杆菌介导的花生遗传转化的原理和方法并对影响转化率的因素进行了探讨。
The review introduced the theory and methods of Agrobacterium-mediated transformation of peanut, and the factors influencing transformation efficiency were discussed.
在进行电击法转化农杆菌时，电场强度和脉冲时间都对农杆菌的转化效率有影响。
While transformation efficiency was influenced by electric field intensity and pulse time when electroporation method was applied.
目前我国在甘蔗遗传转化研究中应用的研究方法主要是农杆菌介导及基因检转化。
The genetic transformation methods for sugarcane in China are mainly Agrogacterium-mediated transformation and gene gun transformation.
农杆菌与愈伤组织混合培养时给予适当的负压条件可提高愈伤组织转化频率和存活率。
The proper negative pressure, when agrobacterium and callus cultivated, could increase transformable frequency and survival rate of callus.
目前外源基因导入玉米受体细胞的方法很多，最受瞩目的是农杆菌介导法和基因枪法。
Agrobacterium and gene gun have aroused most attention among various genetic transformation methods for exogenous gene into maize.
目的：通过农杆菌介导法将白藜芦醇合酶基因转化蔓茎堇菜，并对转化条件进行优化。
Objective: The optimization of the conditions on resveratrol synthase gene transformation for Viola diffusa Ging.
以16种重要的籼稻和粳稻栽培品种为材料，研究了影响农杆菌转化水稻频率的有关因素。
The factors influencing the frequency of rice transformation mediated by Agrobacterium have been investigated by using 16 commercially important indica and japonica rice cultivars or lines.
由于超声波处理可增加农杆菌与外植体之间的接触面积，提高农杆菌对外植体的转化频率。
Sonication assisted Agrobacterium mediated transformation(SAAT)tremendously improves the efficiency of Agrobacterium infection by introducing large numbers of microwounds into the target plant tissue.
通过农杆菌介导，采用叶盘法将豌豆凝集素基因转化单倍体烟草，获得了转基因再生植株。
The pea lectin gene was transformed into the haploid tobacco via Agrobacterium mediation using leaf disc method and the regenerated tobacco plants were obtained.
为了能有效地杀除共培养后残余的农杆菌和筛选转化体，测定了青菜对5种抗生素的敏感性。
For effective elimination of Agrobacterium and screen of transformed cells after co-cultivation, sensitivity of cabbage cultured in vitro to 5 antibiotics was testes.
用农杆菌介导的叶盘法将HAL1基因转入模式植物烟草，对转基因烟草的耐盐性进行评价；
The HAL1 gene was transformed into tobacco by Agrobacterium through leaf disc transformation and the salt tolerance character of transgenic tobacco was analysised.
用农杆菌介导的叶盘转化法将一个来自棉花的谷胱甘肽s转移酶(GST)基因转化到烟草中。
A glutathione S-transferase (GST) gene from cotton was introduced into tobacco by Agrobacturium-mediated leaf disk transformation method.
方法分别用A4、LBA9402和C58C1三种发根农杆菌菌株转化莨菪子叶受体，获得发根；
Methods Hairy root of H. niger was obtained from cotyledon explants after infected with Agrobacterium rhizogenes strains A4, LBA9402, and C58C1, respectively.
目前，农杆菌介导法是苹果上应用的主要转化方法，叶片再生不定芽途径是被广泛应用的受体系统。
At present, Agrobacterium mediated transformation of leaves are widely adopted in apples, and transgenic shoots were mainly obtained from adventitious bud regeneration.
利用基因枪和农杆菌转化法可将大豆铁结合蛋白基因导入旱稻基因组中，并使转基因稻米铁含量增高。
The soybean ferritin gene can be transferred into upland rice using the method of Biolistic and Agrobacterium-mediated transformation, which increased iron concentration in the transgenic plant seeds.
通过对影响根癌农杆菌介导蓝猪耳转化效果各种因素的研究，建立了蓝猪耳高效稳定的遗传转化系统。
An efficient and stable Agrobacterium-mediated genetic transformation system for Torenia was established through investigating the factors influencing the transformation efficiency.
通过对影响根癌农杆菌介导蓝猪耳转化效果各种因素的研究，建立了蓝猪耳高效稳定的遗传转化系统。
An efficient and stable Agrobacterium-mediated genetic transformation system for Torenia was established through investigating the factors influencing the transformation efficiency.
应用推荐