可见，基因型对小麦愈伤组织的分化有很大的影响，附加一定的外源激素有利于提高植株的再生频率。
So the genotypes have great influence on wheat callus differentiation and to add certain amount of hormone could increase the rate of plant regeneration.
因此，选择适当的杂交组合是提高小麦花粉植株再生频率的关键。
It was discussed that cross combination is the key factor to increase the green plantlet regeneration rate of wheat.
用较高渗透压的培养基培养原生质体再生的细胞团及愈伤组织，可提高植株再生频率。
Using media with relatively high osmotic pressure in cul-turing protoplast-derived calli increased their plant regeneration frequency on subsequent differentiation media.
以悬浮细胞作为基因枪轰击的靶材料，转化植株再生频率较初级愈伤组织的为高。
The efficiency of regenerated plant from resistant clones in suspension cells was higher than that in primary callus.
这种变异在植物组织培养后的再生植株中常表现得非常广泛，而且变异频率非常高。
The variation is quite common among regenerated plants after tissue culture, and the frequency of mutation is very high.
因此，研究大麦成熟胚组织培养条件和植株再生频率的影响因素，建立大麦成熟胚高效再生体系对于有效地开展大麦转基因研究具有重要意义。
So it was significant to build an efficient regeneration system for barley mature embryo by studying factors that influence barley mature embryo culture and plant regeneration.
因此，研究大麦成熟胚组织培养条件和植株再生频率的影响因素，建立大麦成熟胚高效再生体系对于有效地开展大麦转基因研究具有重要意义。
So it was significant to build an efficient regeneration system for barley mature embryo by studying factors that influence barley mature embryo culture and plant regeneration.
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