本文报道了利用细菌的全基因组信息和质粒拯救法的原理建立的一种克隆细菌基因组大片段的方法。
To develop a new method to cline large DNA fragment by combining whole genome sequence information and the principle of plasmid rescue.
目的克隆人、黑猩猩和叶猴FKN全基因及体外表达,比较研究FKN在进化过程中基因组水平和蛋白表达水平的差异。
Objective:To clone full-length FKN gene of human, chimpanzee and leaf monkey and compare their difference in genome and protein expression level.
目的克隆人、黑猩猩和叶猴FKN全基因及体外表达,比较研究FKN在进化过程中基因组水平和蛋白表达水平的差异。
Objective:To clone full-length FKN gene of human, chimpanzee and leaf monkey and compare their difference in genome and protein expression level.
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