用免疫蛋白印迹法检测细胞VEGF蛋白表达。
Western blotting was used to detect protein expression of VEGF.
蛋白质印迹法：蛋白质分子从电泳凝胶转移到固相介质，然后用抗体进行免疫检测的技术。
Western blotting --- A technique analogous to Southern blotting, used for detection of proteins, usually by immunological methods.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
肝组织GR采用放射性配体结合分析法测定其最大结合容量以及用免疫印迹法测定其蛋白含量。
Maximal binding volume of glucocorticoid receptor (GR) in hepatic tissue was assayed by radio-ligand binding assay and protein content was assayed by Western blot.
骨形态发生蛋白2与骨桥蛋白检测：采用免疫组织化学染色与蛋白印迹方法。
The changes of BMP2 and osteopontin were detected by immunohistochemistry and Western blotting.
用免疫沉淀和免疫印迹法测定胞浆蛋白酪氨酸磷酸化情况。
By way of immunoprecipitation and immunoblotting, the cytosolic protein tyrosine phosphorylation was examined.
目的：确定蛋白免疫印迹检测大鼠脑组织中P-糖蛋白的最佳实验条件。
Objective: To determine the optimum experiment condition of detecting P—glycoprotein with Western blotting.
结果表明：所获得的重组NP蛋白在ELISA分析中均能与阳性血清和单克隆抗体发生特异性结合，但在免疫印迹分析中仅与阳性血清发生特异性结合。
The results showed recombinant NP protein could bind to both positive serum and MAb with specificity in ELISA, but only bind to positive serum in western blot.
附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行蛋白质的标记和检测很有用。
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
运用免疫荧光和蛋白印迹技术检测了5种食管癌细胞系中NGAL的表达。
The expression level of NGAL in 5 esophageal carcinoma cell lines was also examined by immunofluorescence and western blot.
目的建立人血浆因子V（FV）蛋白免疫印迹分析，对遗传性FV缺乏症家系成员血浆FV含量进行分析。
Objective To develope a western blot of human plasma factor V (FV) and conduct immunoblot analyses on riasma FV from a family with hereditary FV deficiency.
方法利用免疫细胞化学，蛋白质印迹法，RT -PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。
Methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry.
用蛋白质印迹免疫检测技术和免疫细胞化学技术鉴定细胞表型。
Immunocytochemistry and western blotting technology were used to identify the cell phenotype.
利用蛋白质免疫印迹法或免疫电泳方法检查抗体产生情况。
Check the purity of eluted protein with SDS/PAGE and identify the titer of purified antibody with immune electrophoresis.
免疫印迹分析证实目的蛋白与阳性结核病患者抗血清产生特异性反应。
The Western-blotting analysis showed the expressed protein could react with sera from tuberculosis patients specifitally.
免疫印迹杂交结果显示MYOG和MYOD的蛋白质表达水平也发生了与基因表达相一致的变化。
The results of Western blotting showed that protein expression levels of MYOG and MYOD also changed as same as the gene expression levels.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
免疫印迹分析证实目的蛋白与阳性结核病患者抗血清产生特异性反应。
The Western blotting analysis showed the expressed protein could react with the serum from tuberculosis patients speicfically.
通过与甲壳类过敏患者血清的免疫印迹反应，证实融合表达的原肌球蛋白具有过敏原性，表明原肌球蛋白是蟹类的主要过敏原之一。
Western blot analysis of the GST-TM fusion protein using sera from subjects with crustacean allergy confirmed that TM is the major allergen of Chinese mitten crab.
方法 采用免疫组织化学染色法、免疫印迹法，分析检测食管癌标本中EZH2蛋白的表达。
We then detected EZH2 protein expression in the esophageal squamous carcinoma, paracarcinomatous and normal tissues by Western blotting.
利用蛋白免疫印迹及ELISA技术研究重组蛋白与小鼠抗人DR5单克隆抗体AD5-10的结合情况。
The binding ability of these deletant proteins to AD5-10, a mouse anti-human DR5 monoclonal antibody, was evaluated by immunoblotting and ELISA.
研究蛋白质-蛋白质相互作用，由本地和变性凝胶电泳免疫印迹，免疫沉淀。
Study protein-protein interaction by native and denaturing gel electrophoresis, western blotting, and immunology precipitation.
作者表示，该论文滥用和重复使用他们发表的其他论文中的蛋白 免疫印迹图片，违背了《细胞科学期刊》的编辑政策，必须撤销。
The misuse and re-use of western blot bands violated the editorial policy of Journal of Cell Science, and so we must retract this article.
采用单克隆抗体和阳性血清经elisa、阻断elisa、免疫印迹分析重组蛋白的免疫反应性。
Its immunoreactivity was analyzed by Western blot, ELISA and blocking ELISA using MAb and positive serum.
用蛋白质免疫印迹法 （Westernblotting）测定血小板内蛋白酪氨酸磷酸化的表达。
Protein tyrosine phosphorylation in platelets was assayed by Western blotting and platelet aggregation was assayed by nephel omete r.
用蛋白质免疫印迹法 （Westernblotting）测定血小板内蛋白酪氨酸磷酸化的表达。
Protein tyrosine phosphorylation in platelets was assayed by Western blotting and platelet aggregation was assayed by nephel omete r.
应用推荐