依据聚合酶链反应(PCR)和酶联免疫吸附检测(ELISA)的方法目前被应用于主要食源性致病菌的检测。
Polymerase chain reaction-based (PCR-based) and enzyme-linked immunoabsorbent assay (ELISA) methods are now applied in the detection of major food-borne pathogens.
应用反向间接血凝试验（RPHA）、酶免疫测定（EIA）和聚合酶链反应（PCR）测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
方法：应用免疫组织化学和聚合酶链反应—单链构象多态性分析（PCR-SSCP）检测10例舌白斑和32例舌癌，并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
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