筛选出的细胞GFAP免疫细胞化学鉴定阳性。
The alive cells were positive for GFAP immunocytochemistry staining.
免疫细胞化学鉴定出神经干细胞、神经元和胶质细胞。
The nerve stem cells, neurons and glial cells were identified by immunocytochemistry.
结果:(1)经形态学、免疫细胞化学鉴定所培养的细胞为大鼠星形胶质细胞。
Results: (1) the characterization of astrocytes was confirmed based on the morphology and positive by the detection of GFAP antigen.
用免疫细胞化学法进行细胞纯度鉴定。
The purity of these cells was identified by immunohistochemical staining.
培养细胞的观察与鉴定:采用相差显微镜观察和免疫细胞化学方法鉴定。
The observation and identification of the cultured cells: the cells were observed under contrast microscope and identified by immunocytochemistry.
体外培养正常HRPE细胞,角蛋白免疫细胞化学染色鉴定,观察细胞形态。
Normal HRPE cells were cultured in vitro, and were identified by observing the morphous and immunocytochemical staining of keratin.
收集培养至第3周的悬浮细胞,采用免疫细胞化学法进行CD 34单克隆抗体鉴定。
The suspended cells were collected after 3 weeks of culture for anti-CD34 monoclonal antibodies identification by immunocytochemistry.
方法采用组织块培养法培养羊膜细胞,酶消化法对细胞进行传代,应用免疫细胞化学的方法对细胞进行鉴定。
Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
用蛋白质印迹免疫检测技术和免疫细胞化学技术鉴定细胞表型。
Immunocytochemistry and western blotting technology were used to identify the cell phenotype.
以细胞克隆及免疫细胞化学方法判断NSC增殖并鉴定nsc、神经元和神经胶质细胞。结果:两种组织来源细胞在相应培养条件下nsc均有快速增殖。
The identification of the NSC proliferation and the differentiation of the NSC, neurons and glial cells were confirmed by the formation of cells clones and the immunocytochemistry, respectively.
用光镜、免疫细胞化学染色法进行细胞鉴定。
The cells were identified with microscopy and immunohistochemical methods.
通过免疫细胞化学、细胞化学、流式细胞分析法及透射电镜对其分化后细胞进行鉴定。
Cells were characterized by immunohistochemistry, cytochemistry, FACS and ultrastructure to identify and detect the differentiated population and markers.
结论:利用密度梯度离心和贴壁筛选的方法可以分离hmsc,联合应用免疫细胞化学、细胞化学和电镜等技术手段鉴定HMSC的方法有效。
Conclusion The HMSC can be isolated with density-grad centrifugal and anchoring screening. The method to identify HMSC with immunocytochemistry, cytochemistry and electron microscopy is effective.
结论:利用密度梯度离心和贴壁筛选的方法可以分离hmsc,联合应用免疫细胞化学、细胞化学和电镜等技术手段鉴定HMSC的方法有效。
Conclusion The HMSC can be isolated with density-grad centrifugal and anchoring screening. The method to identify HMSC with immunocytochemistry, cytochemistry and electron microscopy is effective.
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