用免疫细胞化学方法分析诱导前后的细胞表型特点。
The characteristics of treated cells were assayed by immunocytochemistry staining analysis.
应用免疫细胞化学方法检测细胞凋亡光密度值情况。
Immunocytochemical method to detect apoptosis situation by optical density.
方法动物行为学观察、免疫细胞化学方法和放射免疫分析法。
Methods Animal behaviour observation, immunocytochemical staining and radioimmunoassay(RIA) were performed.
培养细胞的观察与鉴定：采用相差显微镜观察和免疫细胞化学方法鉴定。
The observation and identification of the cultured cells: the cells were observed under contrast microscope and identified by immunocytochemistry.
用免疫细胞化学方法显示肾上腺神经肽Y（NPY）样细胞体及其纤维的分布。
The localization of NPY-like immunoreactive cell bodies and fibers in rat adrenal gland were observed by immunocytochemical method.
方法：采用SP免疫细胞化学方法，检测70例肝细胞癌组织中P 36的分布和表达。
Methods: SP immunocytochemical techniques were used to detect the distribution and expression of P36 in 70 cases of hepatocellular carcinoma.
超纯正常羊血清在发光和荧光免疫组化和免疫细胞化学方法中作为封闭液和抗体稀释液使用。
Premium quality normal goat serum for use as blocking reagent and antibody diluent for chromogenic and fluorescent immunohistochemical and immunocytochemical assays.
本研究应用电镜免疫细胞化学方法，对小儿先天性巨结肠病结肠壁内含P物质（SP）神经进行了观察。
The ultrastructure of substance P (SP) neuron in the colon of patients with Hirschsprung's disease was investigated by using immunoelectron microscopic method.
方法采用细胞培养技术、免疫细胞化学方法观察新生大鼠皮层神经干细胞向胆碱能神经元定向分化的时空变化。
Observation the space-time change of cortex nerve stem cell in newborn rat orientation differentiation to cholinergic neuron by cell cultured and immunocyte chemistry methods.
免疫细胞化学方法观察了GABAA受体(gabaar)亚单位、GABA和GAD在大鼠三叉神经尾测亚核内的分布及其匹配关系。
The distribution and match relationship of CABAA receptor (GABAAR) subunits and GABA, GAD were observed in the caudal trigeminal nucleus of rat with immunocytochemistry method.
方法用免疫细胞化学方法观察938例甲状腺细针抽吸标本涂片中各种甲状腺疾病的细胞形态及HLA-DR、S-100和CD44蛋白表达。
Methods A morphologic study and HLA DR, S 100 and CD44 expressions by cytochemical and immunocytochemical techniques were performed for cytologic diagnosis of thyroid diseases in 938 samples with FNA.
以细胞克隆及免疫细胞化学方法判断NSC增殖并鉴定nsc、神经元和神经胶质细胞。结果：两种组织来源细胞在相应培养条件下nsc均有快速增殖。
The identification of the NSC proliferation and the differentiation of the NSC, neurons and glial cells were confirmed by the formation of cells clones and the immunocytochemistry, respectively.
本研究用免疫细胞化学方法（ABC法）观察了降钙素基因相关肽（CGRP）在SD大鼠肺中的分布，缺氧对肺中神经内分泌细胞内CGRP含餐的影响。
We observed the distribution of calcitonin gene-related peptide in the SD rat lung and the effect of hypoxia on its quantity by means of immunocytochemistry (ABC method).
用辣根过氧化物酶(hrp)逆行标记与免疫细胞化学相结合方法，在大鼠红核内观察到HRP阳性与P物质反应阳性的双重标记神经元。
Using a combining method of HRP retrograde tracing with immunocytochemistry, HRP and substance P immunoreactivity double-labeled neurons were observed in the red nucleus of rats.
方法采用免疫细胞化学技术显示背根节内nmdar1 (NMDAR1 - LI)阳性细胞数。
Methods The Numbers of NMDAR1-like immunoreactivity (-li) cells in rat lumbar dorsal root ganglia (DRGs) were analysed using immunocytochemistry.
方法采用组织压印片技术和免疫细胞化学染色。
Methods Tissue imprint and immunocytochemical staining were made from the neurons of IMG.
方法利用免疫细胞化学，蛋白质印迹法，RT -PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。
Methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry.
方法：免疫细胞化学双重标记技术。
Methods: The double labeling immunocytochemical staining technique was used.
方法免疫细胞化学技术。
方法P 2x4受体特异性抗体的免疫细胞化学染色。
Method Immunocytochemical staining technique by using specific antibody against P2X4 receptor.
用CB - HRP追踪与免疫细胞化学结合的方法，对大鼠中缝背核接触脑脊液神经元(简称触液神经元)的化学性质进行了研究。
By Using a combined method of CB-HRP tracing with immunocytochemistry, chemical natures of CSF-contacting neurons in the dorsal raphe nucleus were studied.
方法采用组织块培养法培养羊膜细胞，酶消化法对细胞进行传代，应用免疫细胞化学的方法对细胞进行鉴定。
Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
方法采用免疫细胞化学双重标记技术。
Methods Double labeling immunocytochemical staining technique was used.
方法：应用免疫组织化学和免疫细胞化学技术。
METHODS: Technique of immunohistochemistry and immunocytochemistry were used.
方法神经干细胞培养，免疫细胞化学及RT- P CR技术。
Methods Neural stem cell culture, Immunocytochemistry and RT-PCR technique were used.
免疫细胞化学技术、克隆分析等方法评价其生理特性。
Immunocytochemical techniques and clonal analysis were used to assess cellular physiological characteristics.
方法免疫细胞化学和原位杂交技术。
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
方法应用免疫细胞化学和流式细胞术方法。
Methods The immunocytochemical and flow cytometry were used.
方法采用免疫细胞化学双重标记技术。
Methods Immunohistochemical and double immunofluorescence techniques were used in the study.
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