方法：应用免疫组化法检测乳腺癌及相应癌旁组织tlr4、增殖细胞核抗原(pcna)的表达，TUNEL法检测组织中细胞的凋亡。
Methods: the expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method.
方法应用综合系数法对通州市1994～ 1999年计划免疫工作进行评价。
Methods the overall evaluation method was applied to evaluate the immunization work from 1994 to 1999 in Tongzhou city.
方法：应用综合系数法对通州市1999~2003年计划免疫工作进行评价。
Methods:The overall evaluation method was applied to evaluate the immunization work from 1999 to 2003 in TongZhou city.
方法应用酶联免疫法(ELISA)进行诺瓦克病毒抗原检测。
Method Enzyme-linked immunosorbent assay (ELISA) was adopted to determine the antigen of Norwalk virus.
目的探讨双抗体夹心斑点金免疫渗滤法(S -DIGFA)在现场的应用价值。
Objective To explore the application value of double antibody sandwich dot immuno gold filtration assay (s DIGFA) in the field.
目的探讨免疫层析法检测结核抗体在肺结核诊断中的应用价值。
Objective to investigate the application value of immunochromatography for the detection of anti-tuberculosis antibody in diagnosing pulmonary tuberculosis.
对应用免疫荧光法检测鸡痘病毒感染鸡血清进行了研究。
The detection of chicken serum infected with henpox virus using the immunofluorescence method was studied.
从人胼胝中提取角蛋白，制备兔抗人角蛋白抗血清，应用免疫过氧化物酶法，观察了202例人体各种不同肿瘤中角蛋白的分布。
A rabbit antiserum prepared against keratin proteins isolated from human callus was applied to tissue sections of 202 human neoplasms using the immuno-peroxidase techniques.
目的：评价酶免疫分析法（ELISA）体外检测过敏原在荨麻疹病因筛查中的临床应用价值。
Objective: To evaluate the clinical value of in vitro allergen detection by ELISA in aetiological screening of urticaria.
方法对102例急性白血病患者的骨髓或外周血直接涂片，应用13种白细胞系列单克隆抗体，ABC免疫酶标法进行免疫学分型。
Methods The immunophenotyping of 102 cases with AL were detected by 13 leukon monoclonal antibodies with direct smear of peripheral blood or bone marrow and ABC-AP staining method.
目的探讨全自动快速微粒子酶免疫检测法（MEIA）在肝移植中的应用。
Objective To investigate the application of automated microparticle enzyme immunoassays (MEIA) in liver transplantation.
方法应用免疫组化SABC法检测45例人胃癌组织中COX - 2、VEGF的表达，并以30例正常胃黏膜组织作为对照。
Methods The expression of COX-2 and VEGF were studied by immunohistochemistry SABC method in 45 gastric carcinoma specimen and 30 normal gastric tissues as control.
方法应用免疫组织化学SP法检测52例脑膜瘤组织中MMP - 9表达情况，并结合临床病理参数进行综合分析。
Methods the expression of MMP-9 in 52 cases of meningiomas were detected by immunohistochemistry SP method. The clinical and pathological parameters were analyzed at the same time.
方法应用免疫组织化学abc法、神经交点计数法。
Method Immunohistochemical ABC technique and counting number of nerve intersections were used.
方法应用免疫组化PABC法，分别对19例翼状胬肉标本和5例正常结膜组织中的MUC5AC表达进行检测。
Methods The immunohistochemical methods PABC was performed in study of expression of MUC5AC in 19 pterygial specimens and 5 normal conjunctiva.
方法应用酶联免疫吸附试验(ELISA法)，测定44例原发性肺癌、20例肺部良性疾病和20例正常人血清内皮抑素含量。
Methods Serum levels of endostatin were measured by an enzyme-linked immunosorbent assay (ELISA) in 44 patients with lung cancer, 20 patients with benign lung diseases and 20 normal persons.
目的：建立快速测定茶碱血清浓度的高效液相色谱(hplc)法，研究应用此法和常规的荧光偏振免疫分析(FPIA)法测定结果的相关性。
Aim: To establish a rapid HPLC method determining serum theophylline concentration and research the relativity of results determined by HPLC and by routine FPIA.
方法应用放射免疫分析法对某矿山机械厂82名高温作业工人进行ET、CGRP水平的测定。
Methods 82 high temperature exposed workers were studied and their ET and CGRP levels were measured using radio immunity method.
方法应用免疫组化法对101例大肠癌组织石蜡标本进行MAGE-3抗原表达测定。
Method Immunohistochemical technique for MAGE-3 antigen was performed in paraffin embedded sections of 101 CRC specimens.
目的探讨成虫三氯醋酸抽提抗原(AWA - TCA)金标免疫渗滤法在日本血吸虫病诊断和疗效考核中的潜在应用价值。
Objective to investigate the potential of diagnosis and therapeutic efficacy of dot immunogold filtration assay for schistosomiasis japonica using AWA-TCA as antigen.
方法应用免疫荧光法检测60例肺癌患者、15例肺良性肿瘤患者和15例正常健康者外周静脉血T细胞亚群和NK细胞活性；
Methods The T subsets and NK cell from peripheral blood of 60 patients with lung cancer, 15 patients with lung benign tumor and 15 healthy people were detected by immunofluorescence.
方法应用MTT、CFU - L及间接免疫荧光法对18例急性白血病进行观察。
Methods MTT, CFU-L and indirect immune fluorescence were used in 18 patients with acute leukemias.
对手术病理证实的12例恶性肿瘤及12例良性肿瘤的组织切片应用免疫组化SABC法检测VEGF的表达。
SABC immunohistochemical method was used to investigate the expression of VEGF in 12 samples of malignant and 12 samples of benign tumors.
方法应用放射免疫分析法对58例PIH患者、50例晚期妊娠妇女和52例对照组血浆et、CGRP含量进行测定。
Methods: Plasma levels of et, CGRP were measured by radioimmunoassay in 58 patients with PIH (PIH group), 50 normal late-stage pregnant women (pregnant group) and 52 healthy women (control group).
方法应用放射免疫法(RIA)和放射配体受体法。
Methods Radioimmuniassay (RIA) and radioligand receptor assay were used.
应用免疫组织化学ABC法观察各时相点MVZ内孤束核、迷走神经背核FOS蛋白的表达变化规律。
The expression of FOS protein in the nucleus of the solitary and dorsal nucleus of vagus nerve of MVZ were measured by immunohistochemistry(ABC).
方法：应用免疫组织化学SABC法检测41例口腔鳞癌组织中MCP-1的表达，并以10例正常口腔黏膜组织作为对照。
Methods: The expression of MCP-1 in 41 cases of OSCC and 10 specimens of normal oral epithelial tissue was assessed with SABC immunohistochemical method.
方法：应用he和免疫组织化学SABC法染色。
Methods: he staining and immunocytochemistry SABC methods were used.
方法：应用he和免疫组织化学SABC法染色。
Methods: he staining and immunocytochemistry SABC methods were used.
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