经生物素标记探针杂交确认RANTES基因的整合，用免疫斑点印迹法对重组基因表达水平进行定量测定。
The integration of RANTES gene was confirmed by hybridization with biotin-labelled probes and the recombinant gene expression level was quantitatively detected by immunodot blotting.
目的建立一种伴有质控点的金免疫渗滤法（GIFA），用于半定量检测血清甲胎蛋白（AFP）。
Objective To develop a semi-quantitative gold immunofiltration assay(GIFA) with quality control dots for the detection of alpha-fetoprotein(AFP) in serum.
建立的ABC -ELISA定量检测人egf的方法稳定性好，特异性强和灵敏度高，具有替代放射免疫分析法的趋势。
The ABC-ELISA for quantitatively determining human EGF has high stability, specificity and sensitivity. It has the trend to replace radio-immunity analysis.
方法应用免疫组织化学方法检测erk在脊髓后角的定位表达，并用图像分析系统和免疫印迹法进行定量分析。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
方法痰中病原菌定量培养、鉴定及荧光免疫法检测acb及其抗体类型。
METHODS Quantitative culture of pathogenic organisms in sputum samples and immunofluorescence method were used to detect ACBs and their types.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
免疫荧光法和实时定量PCR有较好的一致性（92.3%）。
The correspondence of the HCMV pp65 test and quantitative PCR was 92.3%.
免疫组化法计数VEGF阳性细胞百分数并进行半定量分级。
The VEGF positive cells percentage and semi quantitative classification were obtained by immunohistochemical staining after tumor formation in nude mice.
方法石蜡包埋切片，应用免疫组织化学SP法及麦克奥迪真彩色病理分析图像分析系统，对TTF-1表达强度的阳性单位（PU）进行定量分析。
Methods The TTF-1 expression intensity (in positive units, PU) in paraffin-embedded tissue blocks was determined using immunohistochemical SP method in combination with Motic Images System.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化法和计算机图像分析半定量测定各组大鼠气道降钙素基因相关肽蛋白的含量。
Immunohistochemistry and computer image analysis were used to determine semi-quantitatively the content of CGRP protein in airway of rats.
方法用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测；并用荧光定量PCR法对其进行HBV-DNA检测。
Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.
方法采用免疫组化法对5 3例胰腺癌患者进行IL2R及T细胞定量研究。
Methods IL 2R and T lymphocytes in 53 cases with pancreas carcinoma were examined quantitatively by immunohistochemical method.
方法采用免疫组化法对5 3例胰腺癌患者进行IL2R及T细胞定量研究。
Methods IL 2R and T lymphocytes in 53 cases with pancreas carcinoma were examined quantitatively by immunohistochemical method.
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