利用蛋白质免疫印迹法或免疫电泳方法检查抗体产生情况。
Check the purity of eluted protein with SDS/PAGE and identify the titer of purified antibody with immune electrophoresis.
方法采用免疫组织化学技术、图像分析、放射免疫和免疫印迹法。
Methods Immunohistochemical technique, image analysis, radioimmunoassay and Western blotting were used.
结果：经荧光显微镜、流式细胞仪及免疫印迹法检测，证实AIRE基因得到了稳定表达。
Results: Stable expression of AIRE was confirmed by fluorescence microscope, FACS and western blotting analysis.
方法 采用免疫组织化学染色法、免疫印迹法，分析检测食管癌标本中EZH2蛋白的表达。
We then detected EZH2 protein expression in the esophageal squamous carcinoma, paracarcinomatous and normal tissues by Western blotting.
用蛋白质免疫印迹法 （Westernblotting）测定血小板内蛋白酪氨酸磷酸化的表达。
Protein tyrosine phosphorylation in platelets was assayed by Western blotting and platelet aggregation was assayed by nephel omete r.
方法应用免疫组织化学方法检测erk在脊髓后角的定位表达，并用图像分析系统和免疫印迹法进行定量分析。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
方法：用免疫印迹法检测aif在细胞中的表达量；用免疫组化法检测aif在29例胃癌组织和10例癌旁正常胃组织中的表达。
Immunohistochemical method was used to detect the expression of AIF in 29 cases of gastric cancer tissues and 10 cases of normal gastric mucosa.
目的比较酶联免疫（ELISA）、放射免疫（RIA）和免疫印迹（IB）法检测胰岛素自身抗体（IAA）敏感性和特异性的差别。
Objective To compare the difference of enzyme-linked immunosorbent assay(ELISA), Radioimmunoassay(RIA) and immunoblotting(IB) in the determination of insulin autoantibody(IAA).
方法：采用双向电泳免疫印迹检测法分析了83例CHD患者血清hdl亚类组成及相对百分含量。
Methods: The contents of serum HDL subclasses in 83 CHD subjects were determined by two dimensional gel electrophoresis associated with immunodetection method.
方法用自动分析仪测定生理代谢参数，用免疫印迹和免疫荧光法检测glut1。
Methods Physiological parameters of myocardial metabolism were measured with automated analyzers, and GLUT1 distribution was assessed by immunoblot analysis and immunofluorescence.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
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