结论：杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体，利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
方法：大量样品首先用直接竞争ELISA方法初筛测定，阳性样品再用免疫亲和柱净化、荧光检测器测定的HPLC方法验证和准确定量。
Methods:The concentrations of AFB1 in the samples extracts were initially estimated by ELISA. Samples found to be positive with ELISA were further confirmed and quantified with HPLC.
方法：大量样品首先用直接竞争ELISA方法初筛测定，阳性样品再用免疫亲和柱净化、荧光检测器测定的HPLC方法验证和准确定量。
Methods:The concentrations of AFB1 in the samples extracts were initially estimated by ELISA. Samples found to be positive with ELISA were further confirmed and quantified with HPLC.
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