氯霉素标准品在免疫亲和色谱柱中的添加回收率为99.8%。
The recovery ratio of CAP in immunoaffinity column is 99.8%.
本发明公开了一种自动物样品中提取盐霉素类化合物的方法及其专用免疫亲和吸附剂。
The invention discloses a method for extracting salinomycin compounds from an animal sample and a special immunoaffinity absorbent thereof.
猪肝样品匀浆后用稀盐酸提取,经免疫亲和色谱柱纯化,再用GC/MS检测,即为IAC-GC/MS法。
After extraction with dilute HCl, the extracts of swine liver homogenization was loaded on the IAC column for purification and determined by GC/MS.
结论:杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体,利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
方法:大量样品首先用直接竞争ELISA方法初筛测定,阳性样品再用免疫亲和柱净化、荧光检测器测定的HPLC方法验证和准确定量。
Methods:The concentrations of AFB1 in the samples extracts were initially estimated by ELISA. Samples found to be positive with ELISA were further confirmed and quantified with HPLC.
通过促进初级卫生保健的公平获取来提高卫生的可及性,特别是免疫接种、母亲和儿童健康以及在每一座村庄或城市地区配备一名训练有素的接生员;
increased access to health through promotion of equitable access to primary health care, in particular vaccination, mother and child health and one trained birth attendant per village or city quarter;
多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。抗体用蛋白a和多肽亲和色谱法纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein a and peptide affinity chromatography.
而抗体介导的免疫治疗的关键是靶抗原的特异性强,抗体的免疫原性低及亲和力高等。
The key of antibody-mediated immunological therapy is strong specificity of target antigen, low immunogenicity, high affinity of antibody and so on.
多克隆抗体由合成化肽段免疫动物,该合成肽段与YAP人源蛋白序列一致,抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
目的探讨在不同的免疫学检测方法中,与单链抗体相连的亲和标记肽对单链抗体与抗原结合活性的影响。
Objective to investigate the affects of affinity tag on binding activity of single chain variable fragment (ScFv) to antigen using different immunological assays.
将免疫算法用于求解车辆路径问题,并根据车辆路径问题的具体情况提出了一种基于分组匹配的亲和力计算方法。
This paper proposes a novel affinity based on group match to apply immune algorithm to vehicle routing problem.
该多克隆抗体由合成的人类CDK5氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CDK5. Antibodies are purified by protein A and peptide affinity chromatography.
白细胞介素2 (IL - 2)与其高亲和力受体(IL - 2r)的相互作用在调节T细胞免疫反应的强度和持续时间方面起着关键作用。
The magnitude and duration of T-cell immune response are critically regulated by the interaction of interleukin-2 (IL-2) and its high-affinity receptor (IL-2R) on the surface of the cell.
该多克隆抗体是由合成的人源的针对PRAS40 蛋白氨基酸序列的肽段免疫动物而生产的。抗体由肽亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PRAS40. Antibodies are purified by peptide affinity chromatography.
该多克隆抗体是采用合成的与人源mek2蛋白相一致的合成肽段免疫动物而产生的。该抗体经蛋白a和肽亲和层析纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human MEK2. Antibodies are purified by protein a and peptide affinity chromatography.
该多克隆抗体用与人类PKM2蛋白氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKM2. Antibodies are purified by protein A and peptide affinity chromatography.
多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于人源APC蛋白序列一致,抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human APC. Antibodies are purified by protein a and peptide affinity chromatography.
按这种方法制备的亲和介质提高了纤维素的键合容量,并将在生物产品的大规模分离纯化及人体免疫治疗等方面有广阔的应用前景。
The affinity medium thus prepared has high bonded cellulose capacity and may be widely used in large-scale separation and purification of biological product and body's immunity treatment.
文章评述了这一领域的最新进展,同时也讨论了工程抗体的免疫原性和亲和力。
This review summarized recent developments in this field, also discussed the immunogenicity and affinity of engineered antibodies.
结论抗体抗原亲和力随免疫次数的增加趋向饱和,BSA的线性表位为优势表位,随免疫次数的增加比重逐渐增强。
Conclusion With the increase of the immunization times, the affinity of antibody come to mature and the proportion of dominant linear epitopes of BSA became higher.
该抗体通过使用合成肽段免疫动物而获得,并经蛋白a和肽亲和层析纯化。
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein a and peptide affinity chromatography.
该多克隆抗体是采用合成的与人源mek1蛋白相一致的肽段免疫动物后产生的。该抗体经蛋白a和肽亲和层析纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human MEK1. Antibodies are purified by protein a and peptide affinity chromatography.
该多克隆抗体用与人类LDHA氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA. Antibodies are purified by peptide affinity chromatography.
目的研究亲和免疫吸附清除循环肿瘤坏死因子(TNF)对内毒素性急性肺损伤动物的保护作用。
Objective to study the protective effect of removing circulatory TNF by immunoadsorption on endotoxin induced acute lung injury in rabbits.
该多克隆抗体用与人类己糖激酶i氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白a和肽亲和层析纯化制得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I. antibodies are purified by peptide affinity chromatography.
无机纳米颗粒类非病毒型基因载体因其稳定性好、生物亲和性好、安全、有效、无免疫原性等优点,已引起广泛关注。
Inorganic nanoparticles as non-viral gene carriers have caught much attention because of their good stability, biocompatibility, safety, efficiency and non-immunogenicity.
方法根据刀豆素a对AFP亲和力的不同,采用亲和交叉免疫电泳自显影术检测AFP异质体。
Methods According to the different affinity of ConA with AFP, crossed affinity immunoelectrophoresis autoradiography was used.
应用亲和酶标组化标记法和免疫组化ABC法,测定106例乳腺癌组织中ER、PR和DAKO—M1水平。
The expression of ER, PR and DAKO-M1 in 106 breast carcinomas were examined by bothaffinitive histochemical and ABC technique.
应用亲和酶标组化标记法和免疫组化ABC法,测定106例乳腺癌组织中ER、PR和DAKO—M1水平。
The expression of ER, PR and DAKO-M1 in 106 breast carcinomas were examined by bothaffinitive histochemical and ABC technique.
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