为了便于人们对天花病毒的工作，我对它排序，按不同毒株并制备克隆片段。
We sequenced it and made different strains and prepared clone segments so people could work on it.
你有了某种想要克隆或复制的DNA片段。
You have a fragment of some kind of DNA that you would like to clone or make many copies of.
它被采用并成功地用于将 PCR 片段克隆到多接头中。
It was adopted and successfully applied to clone PCR-fragments into the polylinker.
录像中演示了如何粘帖代码片段，然后用git命令行通过Gist生成的git克隆URL访问粘帖的代码，最后把修改后的版本推送回去。
The screencast shows how to paste snippets then use the generated git clone URL to access the pasted code using command line git and then pushing a modified version back.
克隆到两个胃癌下调的新基因片段。
结果：成功克隆了CAT的基因片段，并在大肠杆菌中得到其融合蛋白的表达。
Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.
目的：克隆测序新分离呼肠病毒的S1全长基因组片段，并对其进行序列分析。
Objective: To clone, sequence and analyze genome segment 1 (S1) of new isolated reovirus strains.
目的克隆表达人MAGE - 3基因片段(210 ~ 623位碱基)，以便研究其对MAGE - 3阳性恶性肿瘤的生物学作用。
Objective: To clone and express the MAGE-3 gene fragment (210 ~ 623nt) for researching its biological effects on MAGE-3 positive malignant tumors.
但是，由于PCR产物的酶切效率很低，致使片段两端不能够被完全切开，最终使得PCR产物的克隆效率较低。
But, the efficency of cloning of PCR product is very poor because of the insufficiently cutting of two points of PCR fragment due to the low cutting efficiency of PCR product.
在获得一定长度片段的序列基础之上利用RACE法可以有效地进行全长的克隆。
RACE could be used to clone the full-length cDNA effectively on the basis of obtaining partial sequence.
为研究该酶，克隆和表达该基因片段。
To study N-acetylglucosaminidase, clone and express the gene.
细菌人工染色体(BAC)是一种承载dna大片段的克隆载体系统，用于人、动物和植物基因组文库构建。
Bacterial artificial chromosome (BAC) is a kind of vector system used to construct large fragment insert libraries of genome DNA in human, animals and plants.
随机挑取约200个阳性克隆进行差异筛选。共获得29个差异片段。
About 200 positive clones selected randomly were differentially screened, and 29 differentially expressed genes were obtained.
结论：获得的差异表达片段为进一步克隆甲状腺癌相关基因及功能研究奠定了良好的基础。
Conclusions: the differentially expressed fragments establish a favorable foundation for cloning genes related to thyroid carcinoma and continuing with their functional studies.
本文综述了野生番茄单片段渐渗系的创建及其在基因精细定位、QTL遗传效应分析以及图位克隆中的应用。
This paper reviews establishment of introgression lines from wild tomato in Lycopersicon by MAS and application of theses lines in fine mapping, analysis of QTL effect and gene map-based cloning.
人类基因组计划是将人类的基因组DNA序列分为大小为150-200碱基片段，装载到定位重叠的细菌克隆中，力求每个克隆序列的准确率大于99.9%。
For Human Genome Project planning digested human gene DNA sequence into many 150-200bp fragments, and reconstructed into the germ clone and to be sure the accuracy is over 99.9%.
本文报道了利用细菌的全基因组信息和质粒拯救法的原理建立的一种克隆细菌基因组大片段的方法。
To develop a new method to cline large DNA fragment by combining whole genome sequence information and the principle of plasmid rescue.
采用RT—PCR方法对口蹄疫病毒O/NY00株基因组L片段进行了分子克隆和序列测定。
The L fragment of the genome of foot-and-mouth disease virus(FMDV) O/NY00 isolate was cloned by RT-PCR, and sequenced.
方法采用RD PCR技术构建SHSY5Y细胞基因片段文库，并对每一个克隆进行测序。
Methods The gene fragment library of SH SY5Y cells was constructed with RD PCR technique.
结论本实验已成功地克隆了人肺癌细胞NHE1基因调控序列中正调控序列片段。
Conclusion The positive regulatory fragment of NHE 1 gene from human lung cancer cells was successfully cloned.
获得了很多差异片段的克隆，并从其中选择3个进行测序。
Gain many clone of gene fragment on differential expression, choice 3 of them for sequencing, and blast the sequence in Genebank.
目的：克隆杜氏盐藻核基质附着区(MAR)结合蛋白片段。
Aim: To clone MAR-binding protein cDNA fragment from Dunaliella salina.
结论：所克隆的序列可能为杜氏盐藻MAR结合蛋白片段。
Conclusion: The cloned sequence is probably MAR binding protein cDNA fragment from Dunaliella salina.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
目的：克隆人牙本质基质蛋白1 (DMP1)成熟肽编码区基因片段。
Aim: Cloning and partially sequencing of human dentin matrix protein 1 (DMP1) encoding mature protein.
克隆出一条新的候选基因片段，为胃印戒细胞癌的病因学研究提供新的线索，为进一步深入研究奠定基础。
An EST of a new given gene was obtained and might be useful in revealing the mechanisms of gastric signet ring cell carcinoma.
目的：克隆及表达人胶质瘤特异性抗原MAGEE1基因片段。
AIM: To clone and express the testicular carcinoma antigen MAGE E1 gene in e.
表明可合并组成一段含启动子、起始密码子和NKND四个氨基酸的678个碱基的单克隆抗体M26～32抗原决定簇基因片段。
A combined 678 bp sequence which containing promoter, initiation codon and NKND sequence can be used for further research of target antigen gene of pan species monoclonal antibody M26~32.
特别的，细胞结合剂是识别并结合CD38蛋白的单 克隆抗体、其表位结合片段。
In particular, the cell binding agent is a monoclonal antibody, and epitope-binding fragments thereof, that recognizes and binds the CD38 protein.
特别的，细胞结合剂是识别并结合CD38蛋白的单 克隆抗体、其表位结合片段。
In particular, the cell binding agent is a monoclonal antibody, and epitope-binding fragments thereof, that recognizes and binds the CD38 protein.
应用推荐