为研究该酶，克隆和表达该基因片段。
To study N-acetylglucosaminidase, clone and express the gene.
目的：克隆和表达人源化小鼠抗人交联纤维蛋白单链抗体。
Objective: To clone and express humanized mouse ScFv against human cross linked fibrin (HScFv).
目的克隆和表达日本血吸虫钙磷蛋白基因，纯化表达产物。
Objective To clone and express Schistosoma japonicum (Sj) calcyphosine gene, and purify the expressed protein.
这是关于美奇酵母属酸性蛋白酶基因克隆和表达的首次研究报导。
All these evidences showed that SAP6 gene cloned in this study indeed encoded the acid protease.
目的克隆和表达一种新的血管形成抑制因子，并研究其抗肿瘤活性。
Objective A new angiogenesis inhibitor HIAF 1 (human inhibitor angiogenesis factor 1) was cloned, expressed in E.
对该基因的克隆和表达分析为今后研究家蚕胚胎发育及细胞周期调控奠定了基础。
The cloning and expression analysis of BmCcnl1 provide the basis for the research of the embryonic development and cell cycle regulation.
目的亚克隆和表达日本血吸虫钙结合蛋白SjE16的编码基因，初步研究其免疫反应性。
Objective To sub-clone and express the gene encoding Schistosoma japonicum calcium-binding protein (SJE16) and study its immunological response.
人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
表观遗传修饰在基因表达和克隆胚的早期发育方面有重要作用。
Epigenetic modifications plays an important role in gene expression and the early development of cloned embryo.
建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
作者介绍了褪黑激素受体1b的结构和功能，褪黑激素受体1b基因的克隆及基因结构、发育性表达与作用、定位与多态性，并讨论了该基因与繁殖季节性的关系。
This review introduced the structure and function of melatonin receptor 1b, the cloning and structure, developmental expression, mapping and polymorphism of melatonin receptor 1b gene.
对花粉发育及其特异基因的克隆、表达和调控进行了综述。
The cloning, expression and controlling of specific genes in developing pollen were reviewed briefly.
目的克隆死亡因子相关基因15（MRG15）并研究其在正常晶状体和年龄相关性白内障晶状体上皮细胞中的表达，比较二者的差异。
ObjectiveTo clone the gene of mortality factor related gene 15(MRG15) and to investigate its expression in lens epithelium cell(LEC) of normal and age related cataractous eyes.
要阐明各种信号传递途径与基因表达调控的机理，克隆和鉴定转录因子是关键。
One of the keys to understand the molecular mechanism of gene expression and the pathway of signal transduction is to clone and identify the transcription factors.
目的：构建血管基膜衍生多功能肽克隆和原核表达载体，并对血管基膜衍生多功能肽氨基酸序列进行空间结构分析预测。
Objective: to construct cloning and prokaryotic expression vector of vascular basement membrane-derived multifunctional peptide and to analyze its space conformation.
随着生物信息学和基因定位的迅猛发展，EST已成为基因定位、基因克隆、基因表达分析的有力工具。
Along with the development of bioinformatics and genetic localization, EST has already become a powerful tool for mapping, cloning and expression profiling of genes.
目的 对弓形虫表面抗原SAG4基因进行克隆、表达和免疫反应性分析。
To clone and express surface antigen SAG4 gene of Toxoplasma gondii, and analyze its immunoreactivity.
方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
文章就高等植物NADP - ME的克隆、功能和基因表达调控方面作一介绍。
This paper is an introduction of the higher plant NADP-ME in the aspect of cloning, function and regulation of gene expression.
目的克隆人、黑猩猩和叶猴FKN全基因及体外表达，比较研究FKN在进化过程中基因组水平和蛋白表达水平的差异。
Objective:To clone full-length FKN gene of human, chimpanzee and leaf monkey and compare their difference in genome and protein expression level.
克隆、表达、纯化和人表皮生长因子在大肠杆菌中的功能研究。
Cloning, expression, purification and functional study of human EGF in E. coli.
本文克隆了赤霉素生物合成途径中关键合成酶：贝壳杉烯氧化酶和20-氧化酶，并研究了二者在果树生长发育中的表达规律。
In this article we cloned two crucial gibberellins biosynthesis enzyme genes: ent - kaurene oxidase and 20 - oxidase, and we investigated their expression patterns. 1.
通过克隆鉴定和序列测定，获得120个有效片段，其中84个认定为序列表达标签（EST）。
Screening and sequencing present 120 valid sequences, 84 of them belong in expressed sequence tags (ESTs).
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
方法采用流式细胞术和特异性标记单克隆抗体，测定内皮细胞表面黏附分子VCAM - 1及CD62E的表达率。
Methods the expression rates of endothelial cell surface adhesion molecules VCAM-1 and CD62E were detected by flow cytometry (FCM) and specific labeled monoclonal antibody.
本文报道了在克隆了小鼠酪氨酸酶基因的基础上，在大肠杆菌表达体系中诱导表达野生型和变异型酪氨酸酶的结果。
Here we reported the cloning of the gene from murine tyrosinase, the expression of wild tyrosinase and variants in E. coli.
目的：克隆人MAGE3基因并进行原核表达和分离纯化。
AIM: to clone human MAGE 3 gene, to induce its prokaryotic expression and to purify the protein.
目的克隆人表皮生长因子受体（HER2）基因，建立共表达HER2基因和雌激素受体（ER）基因的MCF-7细胞模型。
Objective To clone human epidermal growth factor receptor (HER2) gene and establish a MCF-7 cell line with stable co-expression of HER2 and estrogen receptor (ER).
本实验主要内容是克隆和分析在野生型拟南芥表皮毛细胞中特异表达的新的启动子。
Described here is the cloning and characterization of a new trichome-specific-promoter in Arabidopsis.
方法采用LRP单克隆抗体、流式细胞技术分别测定15例单纯缺铁性贫血患者（对照组）和65例AL患者（AL组）LRP的表达率。
The expression rate of LRP was detected by its monoclonal antibody and flow cytometry in 15 asiderotic anemia patients and 65 AL patients.
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