目的:克隆与分析囊虫病诊断用抗原及其编码基因。
AIM: To clone and analyze the cDNA encoding immunodiagnostic antigen of cysticercosis.
克隆与表达弓形虫缓殖子期特异性抗原1(BAG1)的基因,并分析重组抗原的免疫反应性。
Clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
夏春;汪明;朱凌云惠阳胡须鸡干扰素基因分子克隆与序列分析。
Sekellick MJ; Ferrandino'AF; Hopkins DA Chicken interferon gene: cloning, expression, and analysis.
经d NA序列分析表明所获基因含有全部前导序列,其成熟蛋白编码部分与从第一骨架区引物所克隆的序列相符。
DNA sequences analysis indicated that the cloned genes included the whole leader sequences and the mature Ig protein encoding regions.
在解析与优良基因连锁的甲基化位点的基础上,分析一些重要性状如抗旱、抗病、抗虫及产量等性状,并对其基因进行定位,使鉴定和克隆相关基因成为可能。
By analyzing the loci linked to drought tolerance, disease resistance, insect resistance and yield traits, etc, the identification and cloning of related genes is possible.
目的:探讨砷化物与基因的相互作用,克隆砷相关基因,利用生物信息学技术分析克隆基因。
Objective: To explore the interaction of gene with arsenic compounds, arsenic related gene was cloned and its function was analyzed by bioinformatics.
目的克隆与表达弓形虫缓殖子期特异性抗原1(BAG1)的基因,并分析重组抗原的免疫反应性。
Objective To clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
该文以DNA PCR扩增的方法,从拟南芥基因组DNA中克隆出NPR1基因,通过序列分析,所克隆的NPR1 基因与报道的基因序列完全一致。
The NPR1 gene was amplified from Arabidopsis thaliana genome DNA by DNA-PCR method. The DNA sequenced analysis showed that the sequence of amplified NPR1 gene was the same as the published sequence.
目的克隆和分析斯氏按蚊前酚氧化酶基因与约氏疟原虫卵囊黑化关系。
Objective To clone pro-phenoloxidase gene from Anopheles stephensi and to analyze the relationship between PPO1 gene and Plasmodium yoelii oocysts melanization.
方法采用基因克隆、测序及生物信息学技术,分析HLA新等位基因与HLA已知基因序列的差异。
Methods Molecular cloning, sequencing and bioinformatics techniques were used to identify the difference between the new HLA allele and other known alleles.
序列分析表明所克隆的SCF序列与文献报道的一致,构建的表达载体经鉴定正确。
The sequence of the cloned SCF is same as the published report. The construction of expression plasmid is identified in a correct way.
结果表明:所获得的重组NP蛋白在ELISA分析中均能与阳性血清和单克隆抗体发生特异性结合,但在免疫印迹分析中仅与阳性血清发生特异性结合。
The results showed recombinant NP protein could bind to both positive serum and MAb with specificity in ELISA, but only bind to positive serum in western blot.
目的:通过对类风湿性关节炎(RA)小鼠模型发病不同时期的四肢足关节聚集的T细胞克隆型的分析,了解T细胞克隆型与RA病变的关系。
AIM: To understand the relationship between T cell clonotypes and RA invasion through clonotypes of analysis of T cells accumulated in 4 feet joints of SKG mice.
以免疫组化法用纯化蛋白制备的单克隆抗体分析NBEAL1表达与胶质瘤病理分级的相关性。
A NBEAL1 protein specific monoclonal antibody was prepared and was used to study the relationship of NBEAL1 expression with pathological grades of glioma.
利用序列分析技术克隆与抗逆相关的基因,并通过原核表达体系和转基因技术进行功能验证。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
利用序列分析技术克隆与抗逆相关的基因,并通过原核表达体系和转基因技术进行功能验证。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
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