脂质体法转染HEK293细胞，用倒置荧光显微镜检测、分析其在HEK293细胞表达及定位。
The recombinant vector was transfected into HEK293 cells with liposome transfection reagents for transient expression.
分别在移植后第2、4、8、12周4个时间点取脑冷冻切片，在倒置荧光显微镜下观察移植细胞的存活和迁移。
Cryostat sections were cut coronally through the hippocampi of the brains at weeks 2, 4, 8 and 12 after transplantation respectively.
分别在移植后第2、4、8、12周4个时间点取脑冷冻切片，在倒置荧光显微镜下观察移植细胞的存活和迁移。
Cryostat sections were cut coronally through the hippocampi of the brains at weeks 2, 4, 8 and 12 after transplantation respectively.
应用推荐