目的探讨尿中修饰核苷代谢轮廓分析在肺癌诊断中的作用。
Objective To study the feasibility of modified urinary nucleosides metabolic profiling on lung cancer diagnoses.
摘要：目的探讨尿中修饰核苷代谢轮廓分析在肺癌诊断中的作用。
ABSTRACT: Objective To study the feasibility of modified urinary nucleosides metabolic profiling on lung cancer diagnoses.
利用卟啉的1-羟基苯并三氮唑活性酯可将卟啉基团直接修饰在商品化的寡核苷酸片断上。
The porphyrin moiety was modified on the commercial oligonucleotides through the active 1-hydroxybenzotriazole esters of the porphyrins.
一种切除修复方法，通常用来修复经受了大的或不同寻常修饰的核苷酸。
A kind of excision repair usually used to fix nucleotides that have undergone large or unusual modifications.
寡核苷酸固定过程中，末端氨基修饰没有明显的特异性，但是可以提高被固定探针的杂交容量。
During oligonucleotide immobilization, terminal amino modification has no obvious specificity, but it can enhance the hybridization capacity of immobilized oligonucleotides.
与单独使用反义寡核苷酸相比较，萘二酰亚胺对寡核苷酸的共价修饰显著地提高了反义寡核苷酸对KB-A - 1细胞生长的抑制。
Compared with the unmodified one, the inhibition of antisense oligonucleotides to the growth of KB - a - 1 cell was increased by conjugating with naphthyl imide.
经修饰寡核苷酸克服了与当前反义药物有关的许多问题。
The modified oligonucleotides overcome many problems associated with current antisense drugs.
紫外交联对大片段DNA固定率影响显著，但对氨基修饰的寡核苷酸影响不显著；
Ultraviolet crosslinking greatly affected the attachment rate of a large fragment of DNA, but did not noticeably affect the attachment rate of the amino-modified oligonucleotides.
本发明的经修饰寡核苷酸作为治疗剂施用，并且与所针对的RNA分子内的互补序列杂交。
Modified oligonucleotides of the invention are administrated as therapeutic agents, and hybridize to complementary sequences within targeted RNA molecules.
本发明的经修饰寡核苷酸作为治疗剂施用，并且与所针对的RNA分子内的互补序列杂交。
Modified oligonucleotides of the invention are administrated as therapeutic agents, and hybridize to complementary sequences within targeted RNA molecules.
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