载体经修改后包括带有一合成的剪接供体的一个异种内含子， 此供体经促性腺激素a亚单位基因（含有一个剪接受体）的内含子A中某片段的上游改造。
The vector was modified to include a heterologous intron with a synthetic splice donor engineered upstream of a fragment from intron A of the gonadotropin a subunit gene(containing a splice acceptor).
以HPV-18 DNA为供体、小牛胸脉DNA 为载体，NIH3T3细胞为受体细胞，应用磷酸钙沉淀法将供体DNA 引入细胞内，得到了清晰的NIH 3T3细胞形态学转化灶。
NIH 3T3 cells were transfected by human papillomavirus 18(HPV 18). HPV 18 DNA as donor and calf thymus DNA as vector. Calcium phosphate technique was used.
以HPV-18 DNA为供体、小牛胸脉DNA 为载体，NIH3T3细胞为受体细胞，应用磷酸钙沉淀法将供体DNA 引入细胞内，得到了清晰的NIH 3T3细胞形态学转化灶。
NIH 3T3 cells were transfected by human papillomavirus 18(HPV 18). HPV 18 DNA as donor and calf thymus DNA as vector. Calcium phosphate technique was used.
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