目的利用大鼠异位心脏移植模型,探讨门静脉注射供体脾细胞诱导免疫耐受的机制。
Objective on the rat model of heterotopic heart transplantation, the mechanism of immune tolerance induced by portal venous injection of donor spleen cells was studied.
目的:在进行心脏移植手术的同时,经门静脉输注供体脾细胞诱导移植耐受,并探讨其耐受机制。
Objective: to induce cardiac transplantation tolerance in rat by perioperative injection of spleen cells into portal vein, and to study the mechanism concerning transplantation tolerance.
方法在“细胞后环磷酰胺”方法的基础上输注供体脾细胞或去除了T细胞的供体脾细胞,然后进行皮肤移植。观察皮肤存活情况并探讨耐受形成机制。
Methods The donor spleen cells (SPC) or T cell depleted donor SPC were injected into the recipients which had been treated by"cells followed by CP system", after which skin grafting was performed.
方法用荧光标记供体脾淋巴细胞,诱导凋亡后分离纯化。
Methods Allogeneic spleen lymphocytes stained with fluorescein were induced to apoptosis, and then transferred into recipients after purification.
方法用荧光标记供体脾淋巴细胞,诱导凋亡后分离纯化。
Methods Allogeneic spleen lymphocytes stained with fluorescein were induced to apoptosis, and then transferred into recipients after purification.
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